1fnv: Difference between revisions

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-[[Image:1fnv.gif|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_1fnv", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_1fnv|  PDB=1fnv  |  SCENE=  }}
-'''STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A'''
+==STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A==
+<StructureSection load='1fnv' size='340' side='right'caption='[[1fnv]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1fnv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_T12 Streptococcus phage T12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FNV FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fnv OCA], [https://pdbe.org/1fnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fnv RCSB], [https://www.ebi.ac.uk/pdbsum/1fnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fnv ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPEA_STRPY SPEA_STRPY] Causative agent of the symptoms associated with scarlet fever, have been associated with streptococcal toxic shock-like disease and may play a role in the early events of rheumatic fever.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fnv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fnv ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The streptococcal pyrogenic toxins A, B, and C (SPEA, SPEB, and SPEC) are responsible for the fever, rash, and other toxicities associated with scarlet fever and streptococcal toxic shock syndrome. This role, together with the ubiquity of diseases caused by Streptococcus pyogenes, have prompted structural analyses of SPEA by several groups. Papageorgiou et al. (1999) have recently reported the structure of SPEA crystallized in the absence of zinc. Zinc has been shown to be important in the ability of some staphylococcal and streptococcal toxins to stimulate proliferation of CD4+ T-cells. Since cadmium is more electron dense than zinc and typically binds interchangeably, we grew crystals in the presence of 10 mM CdCl2. Crystals have been obtained in three space groups, and the structure in the P2(1)2(1)2(1) crystal form has been refined to 1.9 A resolution. The structural analysis revealed an identical tetramer as well as a novel tetrahedral cluster of cadmium in all three crystal forms on a disulfide loop encompassing residues 87-98. No cadmium was bound at the site homologous to the zinc site in staphylococcal enterotoxins C (SECs) despite the high structural homology between SPEA and SECs. Subsequent soaking of crystals grown in the presence of cadmium in 10 mM ZnCl2 showed that zinc binds in this site (indicating it can discriminate between zinc and cadmium ions) using the three ligands (Asp77, His106, and His110) homologous to the SECs plus a fourth ligand (Glu33).
-==Overview==
+Structure of streptococcal pyrogenic exotoxin A reveals a novel metal cluster.,Earhart CA, Vath GM, Roggiani M, Schlievert PM, Ohlendorf DH Protein Sci. 2000 Sep;9(9):1847-51. PMID:11045630<ref>PMID:11045630</ref>
-The streptococcal pyrogenic toxins A, B, and C (SPEA, SPEB, and SPEC) are responsible for the fever, rash, and other toxicities associated with scarlet fever and streptococcal toxic shock syndrome. This role, together with the ubiquity of diseases caused by Streptococcus pyogenes, have prompted structural analyses of SPEA by several groups. Papageorgiou et al. (1999) have recently reported the structure of SPEA crystallized in the absence of zinc. Zinc has been shown to be important in the ability of some staphylococcal and streptococcal toxins to stimulate proliferation of CD4+ T-cells. Since cadmium is more electron dense than zinc and typically binds interchangeably, we grew crystals in the presence of 10 mM CdCl2. Crystals have been obtained in three space groups, and the structure in the P2(1)2(1)2(1) crystal form has been refined to 1.9 A resolution. The structural analysis revealed an identical tetramer as well as a novel tetrahedral cluster of cadmium in all three crystal forms on a disulfide loop encompassing residues 87-98. No cadmium was bound at the site homologous to the zinc site in staphylococcal enterotoxins C (SECs) despite the high structural homology between SPEA and SECs. Subsequent soaking of crystals grown in the presence of cadmium in 10 mM ZnCl2 showed that zinc binds in this site (indicating it can discriminate between zinc and cadmium ions) using the three ligands (Asp77, His106, and His110) homologous to the SECs plus a fourth ligand (Glu33).
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-FNV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNV OCA].
+</div>
+<div class="pdbe-citations 1fnv" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Structure of streptococcal pyrogenic exotoxin A reveals a novel metal cluster., Earhart CA, Vath GM, Roggiani M, Schlievert PM, Ohlendorf DH, Protein Sci. 2000 Sep;9(9):1847-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11045630 11045630]
+*[[Exotoxin 3D structures|Exotoxin 3D structures]]
-[[Category: Single protein]]
+== References ==
-[[Category: Streptococcus pyogenes]]
+<references/>
-[[Category: Earhart, C A.]]
+__TOC__
-[[Category: Ohlendorf, D H.]]
+</StructureSection>
-[[Category: Roggiani, M.]]
+[[Category: Large Structures]]
-[[Category: Schlievert, P M.]]
+[[Category: Streptococcus phage T12]]
-[[Category: Vath, G M.]]
+[[Category: Earhart CA]]
-[[Category: Exotoxin some]]
+[[Category: Ohlendorf DH]]
-[[Category: Superantigen]]
+[[Category: Roggiani M]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:33:08 2008''
+[[Category: Schlievert PM]]
+[[Category: Vath GM]]