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==SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C==
==SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C==
<StructureSection load='1dl0' size='340' side='right' caption='[[1dl0]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1dl0' size='340' side='right'caption='[[1dl0]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1dl0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DL0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1dl0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DL0 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl0 OCA], [http://pdbe.org/1dl0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dl0 RCSB], [http://www.ebi.ac.uk/pdbsum/1dl0 PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl0 OCA], [https://pdbe.org/1dl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dl0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TK1C_HADVE TK1C_HADVE]] This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.<ref>PMID:10881200</ref> <ref>PMID:17141372</ref> <ref>PMID:18625007</ref> <ref>PMID:18986214</ref>
[https://www.uniprot.org/uniprot/TK1C_HADVE TK1C_HADVE] This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.<ref>PMID:10881200</ref> <ref>PMID:17141372</ref> <ref>PMID:18625007</ref> <ref>PMID:18986214</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 1dl0" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 1dl0" style="background-color:#fffaf0;"></div>
==See Also==
*[[NMR Ensembles of Models|NMR Ensembles of Models]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: King, G F]]
[[Category: Hadronyche versuta]]
[[Category: Wang, X H]]
[[Category: Large Structures]]
[[Category: Atracotoxin]]
[[Category: King GF]]
[[Category: Cystine knot]]
[[Category: Wang XH]]
[[Category: Insecticidal]]
[[Category: Neurotoxin]]
[[Category: Toxin]]
[[Category: Vicinal disulfide]]

Latest revision as of 11:23, 6 November 2024

SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1CSOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C

Structural highlights

1dl0 is a 1 chain structure with sequence from Hadronyche versuta. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR, 20 models
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TK1C_HADVE This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.[1] [2] [3] [4]

Publication Abstract from PubMed

We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.

Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge.,Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:http://dx.doi.org/10.1038/75921
  2. ↑ Tedford HW, Maggio F, Reenan RA, King G. A model genetic system for testing the in vivo function of peptide toxins. Peptides. 2007 Jan;28(1):51-6. Epub 2006 Dec 1. PMID:17141372 doi:http://dx.doi.org/10.1016/j.peptides.2006.08.026
  3. ↑ Gunning SJ, Maggio F, Windley MJ, Valenzuela SM, King GF, Nicholson GM. The Janus-faced atracotoxins are specific blockers of invertebrate K(Ca) channels. FEBS J. 2008 Aug;275(16):4045-59. doi: 10.1111/j.1742-4658.2008.06545.x. Epub, 2008 Jul 9. PMID:18625007 doi:http://dx.doi.org/10.1111/j.1742-4658.2008.06545.x
  4. ↑ Wu Y, Cao G, Pavlicek B, Luo X, Nitabach MN. Phase coupling of a circadian neuropeptide with rest/activity rhythms detected using a membrane-tethered spider toxin. PLoS Biol. 2008 Nov 4;6(11):e273. doi: 10.1371/journal.pbio.0060273. PMID:18986214 doi:http://dx.doi.org/10.1371/journal.pbio.0060273
  5. ↑ Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:http://dx.doi.org/10.1038/75921
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