2g60: Difference between revisions

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-{{Seed}}
-[[Image:2g60.png|left|200px]]
-<!--
+==Structure of anti-FLAG M2 Fab domain==
-The line below this paragraph, containing "STRUCTURE_2g60", creates the "Structure Box" on the page.
+<StructureSection load='2g60' size='340' side='right'caption='[[2g60]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2g60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G60 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g60 OCA], [https://pdbe.org/2g60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g60 RCSB], [https://www.ebi.ac.uk/pdbsum/2g60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g60 ProSAT]</span></td></tr>
-{{STRUCTURE_2g60|  PDB=2g60  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q65ZC0_MOUSE Q65ZC0_MOUSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/2g60_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g60 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The inherent difficulties of stabilizing detergent-solubilized integral membrane proteins for biophysical or structural analysis demand the development of new methodologies to improve success rates. One proven strategy is the use of antibody fragments to increase the ;soluble' portion of any membrane protein, but this approach is limited by the difficulties and expense associated with producing monoclonal antibodies to an appropriate exposed epitope on the target protein. Here, the stabilization of a detergent-solubilized K(+) channel protein, KvPae, by engineering a FLAG-binding epitope into a known loop region of the protein and creating a complex with Fab fragments from commercially available anti-FLAG M2 monoclonal antibodies is reported. Although well diffracting crystals of the complex have not yet been obtained, during the course of crystallization trials the structure of the anti-FLAG M2 Fab domain was solved to 1.86 A resolution. This structure, which should aid future structure-determination efforts using this approach by facilitating molecular-replacement phasing, reveals that the binding pocket appears to be specific only for the first four amino acids of the traditional FLAG epitope, namely DYKD. Thus, the use of antibody fragments for improving the stability of target proteins can be rapidly applied to the study of membrane-protein structure by placing the short DKYD motif within a predicted peripheral loop of that protein and utilizing commercially available anti-FLAG M2 antibody fragments.
-===Structure of anti-FLAG M2 Fab domain===
+Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins.,Roosild TP, Castronovo S, Choe S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):835-9. Epub 2006 Aug 18. PMID:16946459<ref>PMID:16946459</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2g60" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16946459}}, adds the Publication Abstract to the page
+*[[Antibody 3D structures|Antibody 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16946459 is the PubMed ID number.
+*[[Sandbox 20009|Sandbox 20009]]
--->
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-{{ABSTRACT_PUBMED_16946459}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-G60 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G60 OCA].
+</StructureSection>
+[[Category: Large Structures]]
-==Reference==
-Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins., Roosild TP, Castronovo S, Choe S, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):835-9. Epub 2006 Aug 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16946459 16946459]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: Roosild TP]]
-[[Category: Roosild, T P.]]
-[[Category: Anti-flag]]
-[[Category: Antibody]]
-[[Category: Fab domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:08:42 2008''