1jhk: Difference between revisions

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-{{Seed}}
-[[Image:1jhk.png|left|200px]]
-<!--
+==Crystal structure of the anti-estradiol antibody 57-2==
-The line below this paragraph, containing "STRUCTURE_1jhk", creates the "Structure Box" on the page.
+<StructureSection load='1jhk' size='340' side='right'caption='[[1jhk]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1jhk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JHK FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jhk OCA], [https://pdbe.org/1jhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jhk RCSB], [https://www.ebi.ac.uk/pdbsum/1jhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jhk ProSAT]</span></td></tr>
-{{STRUCTURE_1jhk|  PDB=1jhk  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7TS98_MOUSE Q7TS98_MOUSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/1jhk_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jhk ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of a Fab fragment of an anti-17beta-estradiol antibody 57-2 was determined in the absence and presence of the steroid ligand, 17beta-estradiol (E2), at 2.5 and 2.15-A resolutions, respectively. The antibody binds the steroid in a deep hydrophobic pocket formed at the interface between the variable domains. No major structural rearrangements take place upon ligand binding; however, a large part of the heavy chain variable domain near the binding pocket is unusually flexible and is partly stabilized when the steroid is bound. The nonpolar steroid skeleton of E2 is recognized by a number of hydrophobic interactions, whereas the two hydroxyl groups of E2 are hydrogen-bonded to the protein. Especially, the 17-hydroxyl group of E2 is recognized by an intricate hydrogen bonding network in which the 17-hydroxyl itself forms a rare four-center hydrogen bond with three polar amino acids; this hydrogen bonding arrangement accounts for the low cross-reactivity of the antibody with other estrogens such as estrone. The CDRH3 loop plays a prominent role in ligand binding. All the complementarity-determining regions of the light chain make direct contacts with the steroid, even CDRL2, which is rarely directly involved in the binding of haptens.
-===Crystal structure of the anti-estradiol antibody 57-2===
+Crystal structure of a recombinant anti-estradiol Fab fragment in complex with 17beta -estradiol.,Lamminmaki U, Kankare JA J Biol Chem. 2001 Sep 28;276(39):36687-94. Epub 2001 Jul 12. PMID:11451948<ref>PMID:11451948</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_11451948}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1jhk" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 11451948 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_11451948}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-JHK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHK OCA].
-==Reference==
-Crystal structure of a recombinant anti-estradiol Fab fragment in complex with 17beta -estradiol., Lamminmaki U, Kankare JA, J Biol Chem. 2001 Sep 28;276(39):36687-94. Epub 2001 Jul 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11451948 11451948]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: Kankare JA]]
-[[Category: Kankare, J A.]]
+[[Category: Lamminmaki U]]
-[[Category: Lamminmaki, U.]]
-[[Category: Antibody]]
-[[Category: Steroid]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 20:15:21 2008''