1j1o: Difference between revisions

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OCA

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-[[Image:1j1o.gif|left|200px]]<br />
-<applet load="1j1o" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1j1o, resolution 1.8&Aring;" />
-'''Crystal Structure of HyHEL-10 Fv mutant LY50F complexed with hen egg white lysozyme'''<br />
-==Overview==
+==Crystal Structure of HyHEL-10 Fv mutant LY50F complexed with hen egg white lysozyme==
-To study the role of hydrogen bonding via interfacial water molecules in, protein-protein interactions, we examined the interaction between hen egg, white lysozyme (HEL) and its HyHEL-10 variable domain fragment (Fv), antibody. We constructed three antibody mutants (l-Y50F, l-S91A, and, l-S93A) and investigated the interactions between the mutant Fvs and HEL., Isothermal titration calorimetry indicated that the mutations, significantly decreased the negative enthalpy change (8-25 kJ mol(-1)), despite some offset by a favorable entropy change. X-ray crystallography, demonstrated that the complexes had nearly identical structures, including, the positions of the interfacial water molecules. Taken together, the, isothermal titration calorimetric and x-ray crystallographic results, indicate that hydrogen bonding via interfacial water enthalpically, contributes to the Fv-HEL interaction despite the partial offset because, of entropy loss, suggesting that hydrogen bonding stiffens the, antigen-antibody complex.
+<StructureSection load='1j1o' size='340' side='right'caption='[[1j1o]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1j1o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J1O FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j1o OCA], [https://pdbe.org/1j1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j1o RCSB], [https://www.ebi.ac.uk/pdbsum/1j1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j1o ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KV5A9_MOUSE KV5A9_MOUSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/1j1o_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j1o ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+To study the role of hydrogen bonding via interfacial water molecules in protein-protein interactions, we examined the interaction between hen egg white lysozyme (HEL) and its HyHEL-10 variable domain fragment (Fv) antibody. We constructed three antibody mutants (l-Y50F, l-S91A, and l-S93A) and investigated the interactions between the mutant Fvs and HEL. Isothermal titration calorimetry indicated that the mutations significantly decreased the negative enthalpy change (8-25 kJ mol(-1)), despite some offset by a favorable entropy change. X-ray crystallography demonstrated that the complexes had nearly identical structures, including the positions of the interfacial water molecules. Taken together, the isothermal titration calorimetric and x-ray crystallographic results indicate that hydrogen bonding via interfacial water enthalpically contributes to the Fv-HEL interaction despite the partial offset because of entropy loss, suggesting that hydrogen bonding stiffens the antigen-antibody complex.
-==About this Structure==
+The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction.,Yokota A, Tsumoto K, Shiroishi M, Kondo H, Kumagai I J Biol Chem. 2003 Feb 14;278(7):5410-8. Epub 2002 Nov 19. PMID:12444085<ref>PMID:12444085</ref>
-J1O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J1O OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction., Yokota A, Tsumoto K, Shiroishi M, Kondo H, Kumagai I, J Biol Chem. 2003 Feb 14;278(7):5410-8. Epub 2002 Nov 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12444085 12444085]
+</div>
+<div class="pdbe-citations 1j1o" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Gallus gallus]]
-[[Category: Lysozyme]]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: Kondo H]]
-[[Category: Kondo, H.]]
+[[Category: Kumagai I]]
-[[Category: Kumagai, I.]]
+[[Category: Shiroishi M]]
-[[Category: Shiroishi, M.]]
+[[Category: Tsumoto K]]
-[[Category: Tsumoto, K.]]
+[[Category: Yokota A]]
-[[Category: Yokota, A.]]
-[[Category: antigen-antibody complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:33:31 2007''