1axt: Difference between revisions

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-{{Seed}}
-[[Image:1axt.png|left|200px]]
-<!--
+==IMMUNE VERSUS NATURAL SELECTION: ANTIBODY ALDOLASES WITH THE RATES OF NATURAL ENZYMES==
-The line below this paragraph, containing "STRUCTURE_1axt", creates the "Structure Box" on the page.
+<StructureSection load='1axt' size='340' side='right'caption='[[1axt]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1axt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AXT FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1axt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1axt OCA], [https://pdbe.org/1axt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1axt RCSB], [https://www.ebi.ac.uk/pdbsum/1axt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1axt ProSAT]</span></td></tr>
-{{STRUCTURE_1axt|  PDB=1axt  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GCAM_MOUSE GCAM_MOUSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ax/1axt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1axt ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Structural and mechanistic studies show that when the selection criteria of the immune system are changed, catalytic antibodies that have the efficiency of natural enzymes evolve, but the catalytic antibodies are much more accepting of a wide range of substrates. The catalytic antibodies were prepared by reactive immunization, a process whereby the selection criteria of the immune system are changed from simple binding to chemical reactivity. This process yielded aldolase catalytic antibodies that approximated the rate acceleration of the natural enzyme used in glycolysis. Unlike the natural enzyme, however, the antibody aldolases catalyzed a variety of aldol reactions and decarboxylations. The crystal structure of one of these antibodies identified the reactive lysine residue that was selected in the immunization process. This lysine is deeply buried in a hydrophobic pocket at the base of the binding site, thereby accounting for its perturbed pKa.
-===IMMUNE VERSUS NATURAL SELECTION: ANTIBODY ALDOLASES WITH THE RATES OF NATURAL ENZYMES===
+Immune versus natural selection: antibody aldolases with enzymic rates but broader scope.,Barbas CF 3rd, Heine A, Zhong G, Hoffmann T, Gramatikova S, Bjornestedt R, List B, Anderson J, Stura EA, Wilson IA, Lerner RA Science. 1997 Dec 19;278(5346):2085-92. PMID:9405338<ref>PMID:9405338</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_9405338}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1axt" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 9405338 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9405338}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-AXT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AXT OCA].
-==Reference==
-Immune versus natural selection: antibody aldolases with enzymic rates but broader scope., Barbas CF 3rd, Heine A, Zhong G, Hoffmann T, Gramatikova S, Bjornestedt R, List B, Anderson J, Stura EA, Wilson IA, Lerner RA, Science. 1997 Dec 19;278(5346):2085-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9405338 9405338]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: Heine A]]
-[[Category: Heine, A.]]
+[[Category: Wilson IA]]
-[[Category: Wilson, I A.]]
-[[Category: Aldolase reaction]]
-[[Category: Antibody fabs']]
-[[Category: Catalyst]]
-[[Category: Immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:49:23 2008''