2z3q: Difference between revisions

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-[[Image:2z3q.gif|left|200px]]<br /><applet load="2z3q" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2z3q, resolution 1.85&Aring;" />
-'''Crystal structure of the IL-15/IL-15Ra complex'''<br />
-==Overview==
+==Crystal structure of the IL-15/IL-15Ra complex==
-Interleukin 15 (IL-15) and IL-2, which promote the survival of memory, CD8(+) T cells and regulatory T cells, respectively, bind receptor, complexes that share beta- and gamma-signaling subunits. Receptor, specificity is provided by unique, nonsignaling alpha-subunits. Whereas, IL-2 receptor-alpha (IL-2Ralpha) is expressed together in cis with the, beta- and gamma-subunits on T cells and B cells, IL-15Ralpha is expressed, in trans on antigen-presenting cells. Here we present a 1.85-A crystal, structure of the human IL-15-IL-15Ralpha complex. The structure provides, insight into the molecular basis of the specificity of cytokine, recognition and emphasizes the importance of water in generating this very, high-affinity complex. Despite very low IL-15-IL-2 sequence homology and, distinct receptor architecture, the topologies of the IL-15-IL-15Ralpha, and IL-2-IL-2Ralpha complexes are very similar. Our data raise the, possibility that IL-2, like IL-15, might be capable of being presented in, trans in the context of its unique receptor alpha-chain.
+<StructureSection load='2z3q' size='340' side='right'caption='[[2z3q]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2z3q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z3Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z3Q FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z3q OCA], [https://pdbe.org/2z3q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z3q RCSB], [https://www.ebi.ac.uk/pdbsum/2z3q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z3q ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IL15_HUMAN IL15_HUMAN] Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/2z3q_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z3q ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Interleukin 15 (IL-15) and IL-2, which promote the survival of memory CD8(+) T cells and regulatory T cells, respectively, bind receptor complexes that share beta- and gamma-signaling subunits. Receptor specificity is provided by unique, nonsignaling alpha-subunits. Whereas IL-2 receptor-alpha (IL-2Ralpha) is expressed together in cis with the beta- and gamma-subunits on T cells and B cells, IL-15Ralpha is expressed in trans on antigen-presenting cells. Here we present a 1.85-A crystal structure of the human IL-15-IL-15Ralpha complex. The structure provides insight into the molecular basis of the specificity of cytokine recognition and emphasizes the importance of water in generating this very high-affinity complex. Despite very low IL-15-IL-2 sequence homology and distinct receptor architecture, the topologies of the IL-15-IL-15Ralpha and IL-2-IL-2Ralpha complexes are very similar. Our data raise the possibility that IL-2, like IL-15, might be capable of being presented in trans in the context of its unique receptor alpha-chain.
-==About this Structure==
+Crystal structure of the IL-15-IL-15Ralpha complex, a cytokine-receptor unit presented in trans.,Chirifu M, Hayashi C, Nakamura T, Toma S, Shuto T, Kai H, Yamagata Y, Davis SJ, Ikemizu S Nat Immunol. 2007 Sep;8(9):1001-7. Epub 2007 Jul 22. PMID:17643103<ref>PMID:17643103</ref>
-Z3Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z3Q OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of the IL-15-IL-15Ralpha complex, a cytokine-receptor unit presented in trans., Chirifu M, Hayashi C, Nakamura T, Toma S, Shuto T, Kai H, Yamagata Y, Davis SJ, Ikemizu S, Nat Immunol. 2007 Sep;8(9):1001-7. Epub 2007 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17643103 17643103]
+</div>
+<div class="pdbe-citations 2z3q" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Interleukin 3D structures|Interleukin 3D structures]]
+*[[Interleukin receptor 3D structures|Interleukin receptor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Chirifu, M.]]
+[[Category: Chirifu M]]
-[[Category: Davis, S.J.]]
+[[Category: Davis SJ]]
-[[Category: Ikemizu, S.]]
+[[Category: Ikemizu S]]
-[[Category: Yamagata, Y.]]
+[[Category: Yamagata Y]]
-[[Category: cytokine/cytokine receptor complex]]
-[[Category: protein-protein complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:48:20 2008''