1zjk: Difference between revisions

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-[[Image:1zjk.png|left|200px]]
-{{STRUCTURE_1zjk|  PDB=1zjk  |  SCENE=  }}
+==Crystal structure of the zymogen catalytic region of human MASP-2==
+<StructureSection load='1zjk' size='340' side='right'caption='[[1zjk]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1zjk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZJK FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zjk OCA], [https://pdbe.org/1zjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zjk RCSB], [https://www.ebi.ac.uk/pdbsum/1zjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zjk ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/MASP2_HUMAN MASP2_HUMAN] Defects in MASP2 are the cause of MASP2 deficiency (MASPD) [MIM:[https://omim.org/entry/613791 613791]. MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease.<ref>PMID:12904520</ref> <ref>PMID:17252003</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/MASP2_HUMAN MASP2_HUMAN] Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.<ref>PMID:10946292</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/1zjk_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zjk ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Few reports have described in detail a true autoactivation process, where no extrinsic cleavage factors are required to initiate the autoactivation of a zymogen. Herein, we provide structural and mechanistic insight into the autoactivation of a multidomain serine protease: mannose-binding lectin-associated serine protease-2 (MASP-2), the first enzymatic component in the lectin pathway of complement activation. We characterized the proenzyme form of a MASP-2 catalytic fragment encompassing its C-terminal three domains and solved its crystal structure at 2.4 A resolution. Surprisingly, zymogen MASP-2 is capable of cleaving its natural substrate C4, with an efficiency about 10% that of active MASP-2. Comparison of the zymogen and active structures of MASP-2 reveals that, in addition to the activation domain, other loops of the serine protease domain undergo significant conformational changes. This additional flexibility could play a key role in the transition of zymogen MASP-2 into a proteolytically active form. Based on the three-dimensional structures of proenzyme and active MASP-2 catalytic fragments, we present model for the active zymogen MASP-2 complex and propose a mechanism for the autoactivation process.
-===Crystal structure of the zymogen catalytic region of human MASP-2===
+A true autoactivating enzyme. Structural insight into mannose-binding lectin-associated serine protease-2 activations.,Gal P, Harmat V, Kocsis A, Bian T, Barna L, Ambrus G, Vegh B, Balczer J, Sim RB, Naray-Szabo G, Zavodszky P J Biol Chem. 2005 Sep 30;280(39):33435-44. Epub 2005 Jul 21. PMID:16040602<ref>PMID:16040602</ref>
-{{ABSTRACT_PUBMED_16040602}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1zjk" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[1zjk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJK OCA].
+*[[Mannan-binding lectin serine protease|Mannan-binding lectin serine protease]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:016040602</ref><ref group="xtra">PMID:015364579</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Ambrus, G.]]
+[[Category: Large Structures]]
-[[Category: Balczer, J.]]
+[[Category: Ambrus G]]
-[[Category: Barna, L.]]
+[[Category: Balczer J]]
-[[Category: Bian, T.]]
+[[Category: Barna L]]
-[[Category: Gal, P.]]
+[[Category: Bian T]]
-[[Category: Harmat, V.]]
+[[Category: Gal P]]
-[[Category: Kocsis, A.]]
+[[Category: Harmat V]]
-[[Category: Naray-Szabo, G.]]
+[[Category: Kocsis A]]
-[[Category: Sim, R B.]]
+[[Category: Naray-Szabo G]]
-[[Category: Vegh, B.]]
+[[Category: Sim RB]]
-[[Category: Zavodszky, P.]]
+[[Category: Vegh B]]
-[[Category: Beta barrel]]
+[[Category: Zavodszky P]]
-[[Category: Hydrolase]]
-[[Category: Modular protein]]