1zj6: Difference between revisions
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==Crystal structure of human ARL5== | ==Crystal structure of human ARL5== | ||
<StructureSection load='1zj6' size='340' side='right' caption='[[1zj6]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='1zj6' size='340' side='right'caption='[[1zj6]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1zj6]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1zj6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZJ6 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G3D:GUANOSINE-3-MONOPHOSPHATE-5-DIPHOSPHATE'>G3D</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zj6 OCA], [https://pdbe.org/1zj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zj6 RCSB], [https://www.ebi.ac.uk/pdbsum/1zj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zj6 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/ARL5A_HUMAN ARL5A_HUMAN] Lacks ADP-ribosylation enhancing activity (By similarity). | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/1zj6_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/1zj6_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: An | [[Category: Large Structures]] | ||
[[Category: Chang | [[Category: An XM]] | ||
[[Category: Liang | [[Category: Chang WR]] | ||
[[Category: Liu | [[Category: Liang DC]] | ||
[[Category: Shi | [[Category: Liu JF]] | ||
[[Category: Wang | [[Category: Shi L]] | ||
[[Category: Wang ZX]] | |||
Latest revision as of 12:43, 25 December 2024
Crystal structure of human ARL5Crystal structure of human ARL5
Structural highlights
FunctionARL5A_HUMAN Lacks ADP-ribosylation enhancing activity (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedARL5 is a member of ARLs, which is widespread in high eukaryotes and homologous between species. But no structure or biological function of this member is reported. We expressed, purified, and resolved the structure of human ARL5 with bound GDP3'P at 2.0 A resolution. A comparison with the known structures of ARFs shows that besides the typical features of ARFs, human ARL5 has specific features of its own. Bacterially expressed human ARL5 contains bound GDP3'P which is seldom seen in other structures. The hydrophobic tail of the introduced detergent Triton X-305 binds at the possible myristoylation site of Gly2, simulating the myristoylated state of N-terminal amphipathic helix in vivo. The structural features of the nucleotide binding motifs and the switch regions prove that ARL5 will undergo the typical GDP/GTP structural cycle as other members of ARLs, which is the basis of their biological functions. 2.0 A crystal structure of human ARL5-GDP3'P, a novel member of the small GTP-binding proteins.,Wang ZX, Shi L, Liu JF, An XM, Chang WR, Liang DC Biochem Biophys Res Commun. 2005 Jul 8;332(3):640-5. PMID:15896705[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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