1k2a: Difference between revisions

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-[[Image:1k2a.gif|left|200px]]
- {{Structure
+==Modified Form of Eosinophil-derived Neurotoxin==
-|PDB= 1k2a |SIZE=350|CAPTION= <scene name='initialview01'>1k2a</scene>, resolution 1.00&Aring;
+<StructureSection load='1k2a' size='340' side='right'caption='[[1k2a]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+<table><tr><td colspan='2'>[[1k2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K2A FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k2a OCA], [https://pdbe.org/1k2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k2a RCSB], [https://www.ebi.ac.uk/pdbsum/1k2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k2a ProSAT]</span></td></tr>
+</table>
-'''Modified Form of Eosinophil-derived Neurotoxin'''
+== Function ==
+[https://www.uniprot.org/uniprot/RNAS2_HUMAN RNAS2_HUMAN] This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.<ref>PMID:3458170</ref> <ref>PMID:12578357</ref>
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k2a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k2a ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
 The crystal structure of a post-translationally modified form of eosinophil-derived neurotoxin (EDN) with four extra residues on its N terminus ((-4)EDN) has been solved and refined at atomic resolution (1 A). Two of the extra residues can be placed unambiguously, while the density corresponding to two others is poor. The modified N terminus appears to influence the position of the catalytically important His129, possibly explaining the diminished catalytic activity of this variant. However, (-4)EDN has been shown to be cytotoxic to a Kaposi's sarcoma tumor cell line and other endothelial cell lines. Analysis of the structure and function suggests that the reason for cytotoxicity is most likely due to cellular recognition by the N-terminal extension, since the intrinsic activity of the enzyme is not sufficient for cytotoxicity and the N-terminal extension does not affect the conformation of EDN.
-==Disease==
+Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities.,Chang C, Newton DL, Rybak SM, Wlodawer A J Mol Biol. 2002 Mar 15;317(1):119-30. PMID:11916383<ref>PMID:11916383</ref>
-Known disease associated with this structure: High density lipoprotein cholesterol level QTL 7 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131240 131240]]
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-K2A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2A OCA].
+</div>
+<div class="pdbe-citations 1k2a" style="background-color:#fffaf0;"></div>
-==Reference==
+== References ==
-Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities., Chang C, Newton DL, Rybak SM, Wlodawer A, J Mol Biol. 2002 Mar 15;317(1):119-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11916383 11916383]
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Pancreatic ribonuclease]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Chang C]]
-[[Category: Chang, C.]]
+[[Category: Newton DL]]
-[[Category: Newton, D L.]]
+[[Category: Rybak SM]]
-[[Category: Rybak, S M.]]
+[[Category: Wlodawer A]]
-[[Category: Wlodawer, A.]]
-[[Category: SO4]]
-[[Category: rnase a folding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:11:34 2008''