1jwf: Difference between revisions

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-[[Image:1jwf.gif|left|200px]]
-<!--
+==Crystal Structure of human GGA1 VHS domain.==
-The line below this paragraph, containing "STRUCTURE_1jwf", creates the "Structure Box" on the page.
+<StructureSection load='1jwf' size='340' side='right'caption='[[1jwf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1jwf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWF FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwf OCA], [https://pdbe.org/1jwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwf RCSB], [https://www.ebi.ac.uk/pdbsum/1jwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwf ProSAT]</span></td></tr>
-{{STRUCTURE_1jwf|  PDB=1jwf  |  SCENE=  }}
+</table>
+== Function ==
-'''Crystal Structure of human GGA1 VHS domain.'''
+[https://www.uniprot.org/uniprot/GGA1_HUMAN GGA1_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
-==Overview==
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwf ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
 GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-interacting proteins) are critical for the transport of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ADP-ribosylation factor (ARF), and clathrin. The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster dileucine (ACLL) sequences. Here we report the X-ray structure of the GGA1 VHS domain alone, and in complex with the carboxy-terminal peptide of cation-independent mannose 6-phosphate receptor containing an ACLL sequence. The VHS domain forms a super helix with eight alpha-helices, similar to the VHS domains of TOM1 and Hrs. Unidirectional movements of helices alpha6 and alpha8, and some of their side chains, create a set of electrostatic and hydrophobic interactions for correct recognition of the ACLL peptide. This recognition mechanism provides the basis for regulation of protein transport from the TGN to endosomes/lysosomes, which is shared by sortilin and low-density lipoprotein receptor-related protein.
-==About this Structure==
+Structural basis for recognition of acidic-cluster dileucine sequence by GGA1.,Shiba T, Takatsu H, Nogi T, Matsugaki N, Kawasaki M, Igarashi N, Suzuki M, Kato R, Earnest T, Nakayama K, Wakatsuki S Nature. 2002 Feb 21;415(6874):937-41. PMID:11859376<ref>PMID:11859376</ref>
-JWF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWF OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structural basis for recognition of acidic-cluster dileucine sequence by GGA1., Shiba T, Takatsu H, Nogi T, Matsugaki N, Kawasaki M, Igarashi N, Suzuki M, Kato R, Earnest T, Nakayama K, Wakatsuki S, Nature. 2002 Feb 21;415(6874):937-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11859376 11859376]
+</div>
+<div class="pdbe-citations 1jwf" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Earnest, T.]]
+[[Category: Earnest T]]
-[[Category: Igarashi, N.]]
+[[Category: Igarashi N]]
-[[Category: Kato, R.]]
+[[Category: Kato R]]
-[[Category: Kawasaki, M.]]
+[[Category: Kawasaki M]]
-[[Category: Matsugaki, N]]
+[[Category: Matsugaki N]]
-[[Category: Nakayama, K.]]
+[[Category: Nakayama K]]
-[[Category: Nogi, T.]]
+[[Category: Nogi T]]
-[[Category: Shiba, T.]]
+[[Category: Shiba T]]
-[[Category: Suzuki, M.]]
+[[Category: Suzuki M]]
-[[Category: Takatsu, H.]]
+[[Category: Takatsu H]]
-[[Category: Wakatsuki, S.]]
+[[Category: Wakatsuki S]]
-[[Category: Super helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:00:28 2008''