1e1u: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1e1u.png|left|200px]]
-{{STRUCTURE_1e1u|  PDB=1e1u  |  SCENE=  }}
+==Human prion protein variant R220K==
+<StructureSection load='1e1u' size='340' side='right'caption='[[1e1u]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1e1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E1U FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e1u OCA], [https://pdbe.org/1e1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e1u RCSB], [https://www.ebi.ac.uk/pdbsum/1e1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e1u ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/1e1u_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e1u ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The NMR structures of three single-amino acid variants of the C-terminal domain of the human prion protein, hPrP(121-230), are presented. In hPrP(M166V) and hPrP(R220K) the substitution is with the corresponding residue in murine PrP, and in hPrP(S170N) it is with the corresponding Syrian hamster residue. All three substitutions are in the surface region of the structure of the cellular form of PrP (PrP(C)) that is formed by the C-terminal part of helix 3, with residues 218-230, and a loop of residues 166-172. This molecular region shows high species variability and has been implicated in specific interactions with a so far not further characterized "protein X," and it is related to the species barrier for transmission of prion diseases. As expected, the three variant hPrP(121-230) structures have the same global architecture as the previously determined wild-type bovine, human, murine, and Syrian hamster prion proteins, but with the present study two localized "conformational markers" could be related with single amino acid exchanges. These are the length and quality of definition of helix 3, and the NMR-observability of the residues in the loop 166-172. Poor definition of the C-terminal part of helix 3 is characteristic for murine PrP and has now been observed also for hPrP(R220K), and NMR observation of the complete loop 166-172 has so far been unique for Syrian hamster PrP and is now also documented for hPrP(S170N).
-===HUMAN PRION PROTEIN VARIANT R220K===
+NMR structures of three single-residue variants of the human prion protein.,Calzolai L, Lysek DA, Guntert P, von Schroetter C, Riek R, Zahn R, Wuthrich K Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8340-5. PMID:10900000<ref>PMID:10900000</ref>
-{{ABSTRACT_PUBMED_10900000}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1e1u" style="background-color:#fffaf0;"></div>
-[[1e1u]] is a 1 chain structure of [[Prion]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E1U OCA].
 ==See Also==
-*[[Prion|Prion]]
+*[[Prion 3D structures|Prion 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:010900000</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Calzolai, L.]]
+[[Category: Large Structures]]
-[[Category: Guntert, P.]]
+[[Category: Calzolai L]]
-[[Category: Lysek, D A.]]
+[[Category: Guntert P]]
-[[Category: Riek, R.]]
+[[Category: Lysek DA]]
-[[Category: Schroetter, C Von.]]
+[[Category: Riek R]]
-[[Category: Wuthrich, K.]]
+[[Category: Von Schroetter C]]
-[[Category: Zahn, R.]]
+[[Category: Wuthrich K]]
-[[Category: Prion protein]]
+[[Category: Zahn R]]