1adq: Difference between revisions

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-[[Image:1adq.gif|left|200px]]<br />
-<applet load="1adq" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1adq, resolution 3.15&Aring;" />
-'''CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC==
-Rheumatoid factors are the characteristic autoantibodies of rheumatoid, arthritis, which bind to the Fc regions of IgG molecules. Here we report, the crystal structure of the Fab fragment of a patient-derived IgM, rheumatoid factor (RF-AN) complexed with human IgG4 Fc, at 3.2 A, resolution. This is the first structure of an autoantibody-autoantigen, complex. The epitope recognised in IgG Fc includes the C gamma 2/C gamma 3, cleft region, and overlaps the binding sites of bacterial Fc-binding, proteins. The antibody residues involved in autorecognition are all, located at the edge of the conventional combining site surface, leaving, much of the latter available, potentially, for recognition of a different, antigen. Since an important contact residue is somatic mutation, the, structure implicates antigen-driven selection, following somatic mutation, of germline genes, in the production of pathogenic rheumatoid factors.
+<StructureSection load='1adq' size='340' side='right'caption='[[1adq]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1adq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ADQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ADQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1adq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1adq OCA], [https://pdbe.org/1adq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1adq RCSB], [https://www.ebi.ac.uk/pdbsum/1adq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1adq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IGHG4_HUMAN IGHG4_HUMAN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/1adq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1adq ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Rheumatoid factors are the characteristic autoantibodies of rheumatoid arthritis, which bind to the Fc regions of IgG molecules. Here we report the crystal structure of the Fab fragment of a patient-derived IgM rheumatoid factor (RF-AN) complexed with human IgG4 Fc, at 3.2 A resolution. This is the first structure of an autoantibody-autoantigen complex. The epitope recognised in IgG Fc includes the C gamma 2/C gamma 3 cleft region, and overlaps the binding sites of bacterial Fc-binding proteins. The antibody residues involved in autorecognition are all located at the edge of the conventional combining site surface, leaving much of the latter available, potentially, for recognition of a different antigen. Since an important contact residue is somatic mutation, the structure implicates antigen-driven selection, following somatic mutation of germline genes, in the production of pathogenic rheumatoid factors.
-==About this Structure==
+Structure of human IgM rheumatoid factor Fab bound to its autoantigen IgG Fc reveals a novel topology of antibody-antigen interaction.,Corper AL, Sohi MK, Bonagura VR, Steinitz M, Jefferis R, Feinstein A, Beale D, Taussig MJ, Sutton BJ Nat Struct Biol. 1997 May;4(5):374-81. PMID:9145108<ref>PMID:9145108</ref>
-ADQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ADQ OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structure of human IgM rheumatoid factor Fab bound to its autoantigen IgG Fc reveals a novel topology of antibody-antigen interaction., Corper AL, Sohi MK, Bonagura VR, Steinitz M, Jefferis R, Feinstein A, Beale D, Taussig MJ, Sutton BJ, Nat Struct Biol. 1997 May;4(5):374-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9145108 9145108]
+</div>
+<div class="pdbe-citations 1adq" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[3D structures of human antibody|3D structures of human antibody]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Corper, A.L.]]
+[[Category: Corper AL]]
-[[Category: Sutton, B.J.]]
+[[Category: Sutton BJ]]
-[[Category: Taussig, M.J.]]
+[[Category: Taussig MJ]]
-[[Category: complex (immunoglobulin/autoantigen)]]
-[[Category: rheumatoid factor auto-antibody complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:57:48 2007''