2jmg: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:2jmg.png|left|200px]]
-<!--
+==Solution structure of V7R mutant of HIV-1 myristoylated matrix protein==
-The line below this paragraph, containing "STRUCTURE_2jmg", creates the "Structure Box" on the page.
+<StructureSection load='2jmg' size='340' side='right'caption='[[2jmg]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2jmg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMG FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
-{{STRUCTURE_2jmg|  PDB=2jmg  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jmg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmg OCA], [https://pdbe.org/2jmg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jmg RCSB], [https://www.ebi.ac.uk/pdbsum/2jmg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmg ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q6E183_9HIV1 Q6E183_9HIV1]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jmg_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jmg ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+During the late phase of human immunodeficiency virus type-1 (HIV-1) replication, newly synthesized retroviral Gag proteins are targeted to lipid raft regions of specific cellular membranes, where they assemble and bud to form new virus particles. Gag binds preferentially to the plasma membrane (PM) of most hematopoietic cell types, a process mediated by interactions between the cellular PM marker phosphatidylinositol-(4,5)-bisphosphate (PI(4,5)P(2)) and Gag's N-terminally myristoylated matrix (MA) domain. We recently demonstrated that PI(4,5)P(2) binds to a conserved cleft on MA and promotes myristate exposure, suggesting a role as both a direct membrane anchor and myristyl switch trigger. Here we show that PI(4,5)P(2) is also capable of binding to MA proteins containing point mutations that inhibit membrane binding in vitro, and in vivo, including V7R, L8A and L8I. However, these mutants do not exhibit PI(4,5)P(2) or concentration-dependent myristate exposure. NMR studies of V7R and L8A MA reveal minor structural changes that appear to be responsible for stabilizing the myristate-sequestered (myr(s)) species and inhibiting exposure. Unexpectedly, the myristyl group of a revertant mutant with normal PM targeting properties (V7R,L21K) is also tightly sequestered and insensitive to PI(4,5)P(2) binding. This mutant binds PI(4,5)P(2) with twofold higher affinity compared with the native protein, suggesting a potential compensatory mechanism for membrane binding.
-===Solution structure of V7R mutant of HIV-1 myristoylated matrix protein===
+Point mutations in the HIV-1 matrix protein turn off the myristyl switch.,Saad JS, Loeliger E, Luncsford P, Liriano M, Tai J, Kim A, Miller J, Joshi A, Freed EO, Summers MF J Mol Biol. 2007 Feb 16;366(2):574-85. Epub 2006 Dec 1. PMID:17188710<ref>PMID:17188710</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2jmg" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_17188710}}, adds the Publication Abstract to the page
+*[[Gag polyprotein 3D structures|Gag polyprotein 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 17188710 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17188710}}
+__TOC__
+</StructureSection>
-==About this Structure==
-JMG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMG OCA].
-==Reference==
-Point mutations in the HIV-1 matrix protein turn off the myristyl switch., Saad JS, Loeliger E, Luncsford P, Liriano M, Tai J, Kim A, Miller J, Joshi A, Freed EO, Summers MF, J Mol Biol. 2007 Feb 16;366(2):574-85. Epub 2006 Dec 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17188710 17188710]
 [[Category: Human immunodeficiency virus 1]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Freed, E O.]]
+[[Category: Freed EO]]
-[[Category: Joshi, A.]]
+[[Category: Joshi A]]
-[[Category: Kim, A.]]
+[[Category: Kim A]]
-[[Category: Liriano, M.]]
+[[Category: Liriano M]]
-[[Category: Loeliger, E.]]
+[[Category: Loeliger E]]
-[[Category: Luncsford, P.]]
+[[Category: Luncsford P]]
-[[Category: Miller, J.]]
+[[Category: Miller J]]
-[[Category: Saad, J S.]]
+[[Category: Saad JS]]
-[[Category: Summers, M F.]]
+[[Category: Summers MF]]
-[[Category: Tai, J.]]
+[[Category: Tai J]]
-[[Category: V7r mutant of hiv-1 myristoylated matrix protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:21:35 2008''