1ggb: Difference between revisions

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-{{Seed}}
-[[Image:1ggb.png|left|200px]]
-<!--
+==MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY==
-The line below this paragraph, containing "STRUCTURE_1ggb", creates the "Structure Box" on the page.
+<StructureSection load='1ggb' size='340' side='right'caption='[[1ggb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1ggb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GGB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ggb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ggb OCA], [https://pdbe.org/1ggb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ggb RCSB], [https://www.ebi.ac.uk/pdbsum/1ggb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ggb ProSAT]</span></td></tr>
-{{STRUCTURE_1ggb|  PDB=1ggb  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GCAM_MOUSE GCAM_MOUSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gg/1ggb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ggb ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+BACKGROUND: Recent structural results have shown that antibodies use an induced fit mechanism to recognize and bind their antigens. Here we present the crystallographically determined structure of an Fab directed against an HIV-1 peptide (Fab 50.1) in the unliganded state and compare it with the peptide-bound structure. We perform a detailed analysis of the components that contribute to enhanced antigen binding and recognition. RESULTS: Induced fit of Fab 50.1 to its peptide antigen involves a substantial rearrangement of the third complementarity determining region loop of the heavy chain (H3), as well as a large rotation of the variable heavy (VH) chain relative to the variable light (VL) chain. Analysis of other Fab structures suggests that the extent of the surface area buried at the VL-VH interface correlates with the ability to alter antibody quaternary structure by reorientation of the VL-VH domains. CONCLUSION: Fab 50.1 exhibits the largest conformational changes yet observed in a single antibody. These can be attributed to the flexibility of the variable region. Comparisons of new data with previous examples lend to the general conclusion that a small VL-VH interface, due in part to a short H3 loop, permits substantial alterations to the antigen-binding pocket. This has major implications for the prediction, engineering and design of antibody-combining sites.
-===MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY===
+Major antigen-induced domain rearrangements in an antibody.,Stanfield RL, Takimoto-Kamimura M, Rini JM, Profy AT, Wilson IA Structure. 1993 Oct 15;1(2):83-93. PMID:8069628<ref>PMID:8069628</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1ggb" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_8069628}}, adds the Publication Abstract to the page
+*[[Antibody 3D structures|Antibody 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 8069628 is the PubMed ID number.
+*[[Sandbox 20009|Sandbox 20009]]
--->
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-{{ABSTRACT_PUBMED_8069628}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-GGB is a 2 chains structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGB OCA].
+</StructureSection>
+[[Category: Large Structures]]
-==Reference==
+[[Category: Mus musculus]]
-<ref group="xtra">PMID:8069628</ref><references group="xtra"/>
+[[Category: Takimoto-Kamimura M]]
-[[Category: Takimoto-Kamimura, M.]]
+[[Category: Wilson IA]]
-[[Category: Wilson, I A.]]
-[[Category: Immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:27:15 2009''