2c8l: Difference between revisions

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-{{Seed}}
-[[Image:2c8l.png|left|200px]]
-<!--
+==Crystal Structure of (SR) Calcium-ATPase E2(Tg) form==
-The line below this paragraph, containing "STRUCTURE_2c8l", creates the "Structure Box" on the page.
+<StructureSection load='2c8l' size='340' side='right'caption='[[2c8l]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2c8l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C8L FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TG1:[(3S,3aR,4S,6S,6aR,7S,8S,9bS)-6-acetyloxy-4-butanoyloxy-3,3a-dihydroxy-3,6,9-trimethyl-8-[(Z)-2-methylbut-2-enoyl]oxy-2-oxo-4,5,6a,7,8,9b-hexahydroazuleno[4,5-b]furan-7-yl]+octanoate'>TG1</scene></td></tr>
-{{STRUCTURE_2c8l|  PDB=2c8l  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8l OCA], [https://pdbe.org/2c8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c8l RCSB], [https://www.ebi.ac.uk/pdbsum/2c8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8l ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/2c8l_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c8l ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We present crystal structures of the calcium-free E2 state of the sarcoplasmic reticulum Ca2+ -ATPase, stabilized by the inhibitor thapsigargin and the ATP analog AMPPCP. The structures allow us to describe the ATP binding site in a modulatory mode uncoupled from the Asp351 phosphorylation site. The Glu439 side chain interacts with AMPPCP via an Mg2+ ion in accordance with previous Fe2+ -cleavage studies implicating this residue in the ATPase cycle and in magnesium binding. Functional data on Ca2+ mediated activation indicate that the crystallized state represents an initial stage of ATP modulated deprotonation of E2, preceding the binding of Ca2+ ions in the membrane from the cytoplasmic side. We propose a mechanism of Ca2+ activation of phosphorylation leading directly from the compact E2-ATP form to the Ca2E1-ATP state. In addition, a role of Glu439 in ATP modulation of other steps of the functional cycle is suggested.
-===CRYSTAL STRUCTURE OF (SR) CALCIUM-ATPASE E2(TG) FORM===
+Modulatory and catalytic modes of ATP binding by the calcium pump.,Jensen AM, Sorensen TL, Olesen C, Moller JV, Nissen P EMBO J. 2006 Jun 7;25(11):2305-14. Epub 2006 May 18. PMID:16710301<ref>PMID:16710301</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2c8l" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16710301}}, adds the Publication Abstract to the page
+*[[ATPase 3D structures|ATPase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16710301 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16710301}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-C8L is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8L OCA].
-==Reference==
-<ref group="xtra">PMID:16710301</ref><references group="xtra"/>
-[[Category: Calcium-transporting ATPase]]
 [[Category: Oryctolagus cuniculus]]
-[[Category: Jensen, A M.]]
+[[Category: Jensen AM]]
-[[Category: Moller, J V.]]
+[[Category: Moller JV]]
-[[Category: Nissen, P.]]
+[[Category: Nissen P]]
-[[Category: Olesen, C.]]
+[[Category: Olesen C]]
-[[Category: Sorensen, T L.]]
+[[Category: Sorensen TL]]
-[[Category: Atp-binding]]
-[[Category: Ca2+-atpase]]
-[[Category: Calcium transport]]
-[[Category: Cation pump]]
-[[Category: Hydrolase]]
-[[Category: Ion transport]]
-[[Category: Membrane protein]]
-[[Category: Metal-binding]]
-[[Category: Modulatory atp]]
-[[Category: Nucleotide-binding]]
-[[Category: P-type atpase]]
-[[Category: Phosphorylation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:46:10 2009''