1war: Difference between revisions

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-{{Seed}}
-[[Image:1war.png|left|200px]]
-<!--
+==Recombinant Human Purple Acid Phosphatase expressed in Pichia Pastoris==
-The line below this paragraph, containing "STRUCTURE_1war", creates the "Structure Box" on the page.
+<StructureSection load='1war' size='340' side='right'caption='[[1war]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1war]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAR FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-{{STRUCTURE_1war|  PDB=1war  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1war FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1war OCA], [https://pdbe.org/1war PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1war RCSB], [https://www.ebi.ac.uk/pdbsum/1war PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1war ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/PPA5_HUMAN PPA5_HUMAN] Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI) [MIM:[https://omim.org/entry/607944 607944]. A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. Note=ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease.<ref>PMID:21217755</ref> <ref>PMID:21217752</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/PPA5_HUMAN PPA5_HUMAN] Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1war_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1war ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of human purple acid phosphatase recombinantly expressed in Escherichia coli (rHPAP(Ec)) and Pichia pastoris (rHPAP(Pp)) has been determined in two different crystal forms, both at 2.2A resolution. In both cases, the enzyme crystallized in its oxidized (inactive) state, in which both Fe atoms in the dinuclear active site are Fe(III). The main difference between the two structures is the conformation of the enzyme "repression loop". Proteolytic cleavage of this loop in vivo or in vitro results in significant activation of the mammalian PAPs. In the crystals obtained from rHPAP(Ec), the carboxylate side-chain of Asp145 of this loop acts as a bidentate ligand that bridges the two metal atoms, in a manner analogous to a possible binding mode for a phosphate ester substrate in the enzyme-substrate complex. The carboxylate side-chain of Asp145 and the neighboring Phe146 side-chain thus block the active site, thereby inactivating the enzyme. In the crystal structure of rHPAP(Pp), the enzyme "repression loop" has an open conformation similar to that observed in other mammalian PAP structures. The present structures demonstrate that the repression loop exhibits significant conformational flexibility, and the observed alternate binding mode suggests a possible inhibitory role for this loop.
-===RECOMBINANT HUMAN PURPLE ACID PHOSPHATASE EXPRESSED IN PICHIA PASTORIS===
+Crystal structures of recombinant human purple Acid phosphatase with and without an inhibitory conformation of the repression loop.,Strater N, Jasper B, Scholte M, Krebs B, Duff AP, Langley DB, Han R, Averill BA, Freeman HC, Guss JM J Mol Biol. 2005 Aug 5;351(1):233-46. Epub 2005 Apr 26. PMID:15993892<ref>PMID:15993892</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1war" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_15993892}}, adds the Publication Abstract to the page
+*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 15993892 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15993892}}
+__TOC__
+</StructureSection>
-==About this Structure==
-WAR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAR OCA].
-==Reference==
-<ref group="xtra">PMID:15993892</ref><references group="xtra"/>
-[[Category: Acid phosphatase]]
 [[Category: Homo sapiens]]
-[[Category: Averill, B A.]]
+[[Category: Large Structures]]
-[[Category: Duff, A P.]]
+[[Category: Averill BA]]
-[[Category: Freeman, H C.]]
+[[Category: Duff AP]]
-[[Category: Guss, J M.]]
+[[Category: Freeman HC]]
-[[Category: Han, R.]]
+[[Category: Guss JM]]
-[[Category: Langley, D B.]]
+[[Category: Han R]]
-[[Category: Glycoprotein]]
+[[Category: Langley DB]]
-[[Category: Hydrolase]]
-[[Category: Iron]]
-[[Category: Iron transport]]
-[[Category: Metalloenzyme]]
-[[Category: Purple acid phosphatase]]
-[[Category: Signal]]
-[[Category: Tartrate resistant acid phosphatase]]
-[[Category: Uteroferrin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 16:38:56 2009''