1usx: Difference between revisions

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-{{Seed}}
-[[Image:1usx.png|left|200px]]
-<!--
+==Crystal structure of the Newcastle disease virus hemagglutinin-neuraminidase complexed with thiosialoside==
-The line below this paragraph, containing "STRUCTURE_1usx", creates the "Structure Box" on the page.
+<StructureSection load='1usx' size='340' side='right'caption='[[1usx]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1usx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Avian_orthoavulavirus_1 Avian orthoavulavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1USX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1USX FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAN:2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC+ACID'>DAN</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=WIA:METHYL(6S)-1-THIO-L-MANNO-HEXODIALDO-6,2-PYRANOSIDE'>WIA</scene></td></tr>
-{{STRUCTURE_1usx|  PDB=1usx  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1usx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1usx OCA], [https://pdbe.org/1usx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1usx RCSB], [https://www.ebi.ac.uk/pdbsum/1usx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1usx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HN_NDVB HN_NDVB] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).  Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/us/1usx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1usx ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Paramyxoviruses are the leading cause of respiratory disease in children. Several paramyxoviruses possess a surface glycoprotein, the hemagglutinin-neuraminidase (HN), that is involved in attachment to sialic acid receptors, promotion of fusion, and removal of sialic acid from infected cells and progeny virions. Previously we showed that Newcastle disease virus (NDV) HN contained a pliable sialic acid recognition site that could take two states, a binding state and a catalytic state. Here we present evidence for a second sialic acid binding site at the dimer interface of HN and present a model for its involvement in cell fusion. Three different crystal forms of NDV HN now reveal identical tetrameric arrangements of HN monomers, perhaps indicative of the tetramer association found on the viral surface.
-===CRYSTAL STRUCTURE OF THE NEWCASTLE DISEASE VIRUS HEMAGGLUTININ-NEURAMINIDASE COMPLEXED WITH THIOSIALOSIDE===
+Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion.,Zaitsev V, von Itzstein M, Groves D, Kiefel M, Takimoto T, Portner A, Taylor G J Virol. 2004 Apr;78(7):3733-41. PMID:15016893<ref>PMID:15016893</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1usx" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_15016893}}, adds the Publication Abstract to the page
+*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 15016893 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15016893}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Avian orthoavulavirus 1]]
-USX is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Newcastle_disease_virus Newcastle disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1USX OCA].
+[[Category: Large Structures]]
+[[Category: Groves D]]
-==Reference==
+[[Category: Itzstein M]]
-<ref group="xtra">PMID:15016893</ref><references group="xtra"/>
+[[Category: Kiefel M]]
-[[Category: Exo-alpha-sialidase]]
+[[Category: Portner A]]
-[[Category: Newcastle disease virus]]
+[[Category: Takimoto T]]
-[[Category: Groves, D.]]
+[[Category: Taylor G]]
-[[Category: Itzstein, M Von.]]
+[[Category: Zaitsev V]]
-[[Category: Kiefel, M.]]
-[[Category: Portner, A.]]
-[[Category: Takimoto, T.]]
-[[Category: Taylor, G.]]
-[[Category: Zaitsev, V.]]
-[[Category: Hemagglutinin]]
-[[Category: Hemagglutinin-neuraminidase]]
-[[Category: Hydrolase]]
-[[Category: Neuraminidase]]
-[[Category: Sialidase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:33:35 2009''