2c4b: Difference between revisions

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-{{Seed}}
-[[Image:2c4b.png|left|200px]]
-<!--
+==Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A==
-The line below this paragraph, containing "STRUCTURE_2c4b", creates the "Structure Box" on the page.
+<StructureSection load='2c4b' size='340' side='right'caption='[[2c4b]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens], [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [https://en.wikipedia.org/wiki/Momordica_cochinchinensis Momordica cochinchinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C4B FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2c4b|  PDB=2c4b  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [https://pdbe.org/2c4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB], [https://www.ebi.ac.uk/pdbsum/2c4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c4b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.[https://www.uniprot.org/uniprot/ITR2_ECBEL ITR2_ECBEL] Inhibits trypsin.[https://www.uniprot.org/uniprot/ITR2_MOMCO ITR2_MOMCO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/2c4b_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c4b ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.
-===INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A===
+Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI.,Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652<ref>PMID:16337652</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2c4b" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16337652}}, adds the Publication Abstract to the page
+*[[Barnase 3D structures|Barnase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16337652 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16337652}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Bacillus amyloliquefaciens]]
-C4B is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens,_momordica_cochinchinensis,_ecballium_elaterium Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA].
+[[Category: Ecballium elaterium]]
+[[Category: Large Structures]]
-==Reference==
+[[Category: Momordica cochinchinensis]]
-<ref group="xtra">PMID:16337652</ref><references group="xtra"/>
+[[Category: Heinz DW]]
-[[Category: Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]]
+[[Category: Kolmar H]]
-[[Category: Heinz, D W.]]
+[[Category: Niemann HH]]
-[[Category: Kolmar, H.]]
+[[Category: Schmoldt HU]]
-[[Category: Niemann, H H.]]
+[[Category: Wentzel A]]
-[[Category: Schmoldt, H U.]]
-[[Category: Wentzel, A.]]
-[[Category: Endonuclease]]
-[[Category: Fusion protein]]
-[[Category: Hybrid microprotein]]
-[[Category: Hydrolase]]
-[[Category: Nuclease]]
-[[Category: Protease inhibitor]]
-[[Category: Ribonuclease]]
-[[Category: Serine protease inhibitor]]
-[[Category: Squash inhibitor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:15:55 2009''