1gxu: Difference between revisions

 

==Hydrogenase Maturation Protein HypF "acylphosphatase-like" N-terminal domain (HypF-ACP) in complex with a substrate. Crystal grown in the presence of carbamoylphosphate==

<StructureSection load='1gxu' size='340' side='right'caption='[[1gxu]], [[Resolution|resolution]] 1.27&Aring;' scene=''>

<table><tr><td colspan='2'>[[1gxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GXU FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27&#8491;</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gxu OCA], [https://pdbe.org/1gxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gxu RCSB], [https://www.ebi.ac.uk/pdbsum/1gxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gxu ProSAT]</span></td></tr>

[https://www.uniprot.org/uniprot/HYPF_ECOLI HYPF_ECOLI] Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.<ref>PMID:8661925</ref> <ref>PMID:12377778</ref> <ref>PMID:15291820</ref> <ref>PMID:15504408</ref>  

     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/1gxu_consurf.spt"</scriptWhenChecked>

     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gxu ConSurf].

*[[HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures|HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures]]

[[Category: Escherichia coli]]

[[Category: Large Structures]]

[[Category: Bolognesi M]]

[[Category: Bucciantini M]]

[[Category: Ramponi G]]

[[Category: Rosano C]]

[[Category: Stefani M]]

[[Category: Zuccotti S]]