2uz6: Difference between revisions

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-{{Seed}}
-[[Image:2uz6.png|left|200px]]
-<!--
+==AChBP-targeted a-conotoxin correlates distinct binding orientations with nAChR subtype selectivity.==
-The line below this paragraph, containing "STRUCTURE_2uz6", creates the "Structure Box" on the page.
+<StructureSection load='2uz6' size='340' side='right'caption='[[2uz6]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2uz6]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica] and [https://en.wikipedia.org/wiki/Conus_textile Conus textile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UZ6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-{{STRUCTURE_2uz6|  PDB=2uz6  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uz6 OCA], [https://pdbe.org/2uz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2uz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uz6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uz/2uz6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uz6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Neuronal nAChRs are a diverse family of pentameric ion channels with wide distribution throughout cells of the nervous and immune systems. However, the role of specific subtypes in normal and pathological states remains poorly understood due to the lack of selective probes. Here, we used a binding assay based on acetylcholine-binding protein (AChBP), a homolog of the nicotinic acetylcholine ligand-binding domain, to discover a novel alpha-conotoxin (alpha-TxIA) in the venom of Conus textile. Alpha-TxIA bound with high affinity to AChBPs from different species and selectively targeted the alpha(3)beta(2) nAChR subtype. A co-crystal structure of Ac-AChBP with the enhanced potency analog TxIA(A10L), revealed a 20 degrees backbone tilt compared to other AChBP-conotoxin complexes. This reorientation was coordinated by a key salt bridge formed between Arg5 (TxIA) and Asp195 (Ac-AChBP). Mutagenesis studies, biochemical assays and electrophysiological recordings directly correlated the interactions observed in the co-crystal structure to binding affinity at AChBP and different nAChR subtypes. Together, these results establish a new pharmacophore for the design of novel subtype-selective ligands with therapeutic potential in nAChR-related diseases.
-===ACHBP-TARGETED A-CONOTOXIN CORRELATES DISTINCT BINDING ORIENTATIONS WITH NACHR SUBTYPE SELECTIVITY.===
+AChBP-targeted alpha-conotoxin correlates distinct binding orientations with nAChR subtype selectivity.,Dutertre S, Ulens C, Buttner R, Fish A, van Elk R, Kendel Y, Hopping G, Alewood PF, Schroeder C, Nicke A, Smit AB, Sixma TK, Lewis RJ EMBO J. 2007 Aug 22;26(16):3858-67. Epub 2007 Jul 26. PMID:17660751<ref>PMID:17660751</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2uz6" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_17660751}}, adds the Publication Abstract to the page
+*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 17660751 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17660751}}
+__TOC__
+</StructureSection>
-==About this Structure==
-UZ6 is a 20 chains structure of sequences from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UZ6 OCA].
-==Reference==
-<ref group="xtra">PMID:17660751</ref><references group="xtra"/>
 [[Category: Aplysia californica]]
-[[Category: Alewood, P F.]]
+[[Category: Conus textile]]
-[[Category: Buttner, R.]]
+[[Category: Large Structures]]
-[[Category: Dutertre, S.]]
+[[Category: Alewood PF]]
-[[Category: Elk, R Van.]]
+[[Category: Buttner R]]
-[[Category: Fish, A.]]
+[[Category: Dutertre S]]
-[[Category: Hopping, G.]]
+[[Category: Fish A]]
-[[Category: Kendel, Y.]]
+[[Category: Hopping G]]
-[[Category: Lewis, R J.]]
+[[Category: Kendel Y]]
-[[Category: Nicke, A.]]
+[[Category: Lewis RJ]]
-[[Category: Schroeder, C.]]
+[[Category: Nicke A]]
-[[Category: Sixma, T K.]]
+[[Category: Schroeder C]]
-[[Category: Smit, A B.]]
+[[Category: Sixma TK]]
-[[Category: Ulens, C.]]
+[[Category: Smit AB]]
-[[Category: Receptor]]
+[[Category: Ulens C]]
-[[Category: Soluble acetylcholine receptor]]
+[[Category: Van Elk R]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:56:44 2009''