2je2: Difference between revisions

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-[[Image:2je2.jpg|left|200px]]<br /><applet load="2je2" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2je2, resolution 1.80&Aring;" />
-'''CYTOCHROME P460 FROM NITROSOMONAS EUROPAEA- PROBABLE NONPHYSIOLOGICAL OXIDIZED FORM'''<br />
-==Overview==
+==Cytochrome P460 from Nitrosomonas europaea - probable nonphysiological oxidized form==
-We have determined the 1.8 A X-ray crystal structure of a monoheme c-type, cytochrome, cytochrome P460, from Nitrosomonas europea. The chromophore, possesses unusual spectral properties analogous to those of the catalytic, heme P460 of hydroxylamine oxidoreductase (HAO), the only known heme in, biology to withdraw electrons from an iron-coordinated substrate. The, analysis reveals a homodimeric structure and elucidates a new c-type, cytochrome fold that is predominantly beta-sheet. In addition to the two, cysteine thioether links to the porphyrin typical of c-type hemes, there, is a third proteinaceous link involving a conserved lysine. The covalent, bond is between the lysine side-chain nitrogen and the 13'-meso carbon of, the heme, which, following cross-link formation, is sp3-hybridized, demonstrating the loss of conjugation at this position within the, porphyrin. The structure has implications for the analogous tyrosine-heme, meso carbon cross-link observed in HAO.
+<StructureSection load='2je2' size='340' side='right'caption='[[2je2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2je2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JE2 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2je2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je2 OCA], [https://pdbe.org/2je2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2je2 RCSB], [https://www.ebi.ac.uk/pdbsum/2je2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2je2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q50927_NITER Q50927_NITER]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2je2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2je2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have determined the 1.8 A X-ray crystal structure of a monoheme c-type cytochrome, cytochrome P460, from Nitrosomonas europea. The chromophore possesses unusual spectral properties analogous to those of the catalytic heme P460 of hydroxylamine oxidoreductase (HAO), the only known heme in biology to withdraw electrons from an iron-coordinated substrate. The analysis reveals a homodimeric structure and elucidates a new c-type cytochrome fold that is predominantly beta-sheet. In addition to the two cysteine thioether links to the porphyrin typical of c-type hemes, there is a third proteinaceous link involving a conserved lysine. The covalent bond is between the lysine side-chain nitrogen and the 13'-meso carbon of the heme, which, following cross-link formation, is sp3-hybridized, demonstrating the loss of conjugation at this position within the porphyrin. The structure has implications for the analogous tyrosine-heme meso carbon cross-link observed in HAO.
-==About this Structure==
+The crystal structure of cytochrome P460 of Nitrosomonas europaea reveals a novel cytochrome fold and heme-protein cross-link.,Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:17583915<ref>PMID:17583915</ref>
-JE2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=HEC:'>HEC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Po4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE2 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-The Crystal Structure of Cytochrome P460 of Nitrosomonas europaea Reveals a Novel Cytochrome Fold and Heme-Protein Cross-link(,)., Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM, Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17583915 17583915]
+</div>
+<div class="pdbe-citations 2je2" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Nitrosomonas europaea]]
-[[Category: Single protein]]
+[[Category: Elmore BO]]
-[[Category: Elmore, B.O.]]
+[[Category: Ferrara JD]]
-[[Category: Ferrara, J.D.]]
+[[Category: Hooper AB]]
-[[Category: Hooper, A.B.]]
+[[Category: Pearson AR]]
-[[Category: Pearson, A.R.]]
+[[Category: Wilmot CM]]
-[[Category: Wilmot, C.M.]]
+[[Category: Yang C]]
-[[Category: Yang, C.]]
-[[Category: HEC]]
-[[Category: PO4]]
-[[Category: cross-linked heme]]
-[[Category: cytochrome p460]]
-[[Category: heme p460]]
-[[Category: metal binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:28:35 2008''