2ccv: Difference between revisions

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-[[Image:2ccv.gif|left|200px]]
-<!--
+==Structure of Helix Pomatia agglutinin with zinc and N-acetyl-alpha-D- galactoseamine (GalNAc)==
-The line below this paragraph, containing "STRUCTURE_2ccv", creates the "Structure Box" on the page.
+<StructureSection load='2ccv' size='340' side='right'caption='[[2ccv]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CCV FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_2ccv|  PDB=2ccv  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ccv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccv OCA], [https://pdbe.org/2ccv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ccv RCSB], [https://www.ebi.ac.uk/pdbsum/2ccv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ccv ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/2ccv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ccv ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Helix pomatia agglutinin (HPA) is a N-acetylgalactosamine (GalNAc) binding lectin found in the albumen gland of the roman snail. As a constituent of perivitelline fluid, HPA protects fertilized eggs from bacteria and is part of the innate immunity system of the snail. The peptide sequence deduced from gene cloning demonstrates that HPA belongs to a family of carbohydrate-binding proteins recently identified in several invertebrates. This domain is also present in discoidin from the slime mold Dictyostelium discoideum. Investigation of the lectin specificity was performed with the use of glycan arrays, demonstrating that several GalNAc-containing oligosaccharides are bound and rationalizing the use of this lectin as a cancer marker. Titration microcalorimetry performed on the interaction between HPA and GalNAc indicates an affinity in the 10(-4) M range with an enthalpy-driven binding mechanism. The crystal structure of HPA demonstrates the occurrence of a new beta-sandwich lectin fold. The hexameric quaternary state was never observed previously for a lectin. The high resolution structure complex of HPA with GalNAc characterizes a new carbohydrate binding site and rationalizes the observed preference for alphaGalNAc-containing oligosaccharides.
-'''STRUCTURE OF HELIX POMATIA AGGLUTININ WITH ZINC AND N-ACETYL-ALPHA-D-GALACTOSEAMINE (GALNAC)'''
+Biochemical and structural analysis of Helix pomatia agglutinin. A hexameric lectin with a novel fold.,Sanchez JF, Lescar J, Chazalet V, Audfray A, Gagnon J, Alvarez R, Breton C, Imberty A, Mitchell EP J Biol Chem. 2006 Jul 21;281(29):20171-80. Epub 2006 May 16. PMID:16704980<ref>PMID:16704980</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ccv" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-CCV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCV OCA].
+*[[Agglutinin 3D structures|Agglutinin 3D structures]]
-[[Category: Helix pomatia]]
+== References ==
-[[Category: Single protein]]
+<references/>
-[[Category: Audfray, A.]]
+__TOC__
-[[Category: Breton, C.]]
+</StructureSection>
-[[Category: Chazalet, V.]]
+[[Category: Large Structures]]
-[[Category: Gagnon, J.]]
+[[Category: Audfray A]]
-[[Category: Gautier, C.]]
+[[Category: Breton C]]
-[[Category: Imberty, A.]]
+[[Category: Chazalet V]]
-[[Category: Lescar, J.]]
+[[Category: Gagnon J]]
-[[Category: Mitchell, E.]]
+[[Category: Gautier C]]
-[[Category: Sanchez, J F.]]
+[[Category: Imberty A]]
-[[Category: Helix pomatia]]
+[[Category: Lescar J]]
-[[Category: Lectin]]
+[[Category: Mitchell EP]]
-[[Category: Snail]]
+[[Category: Sanchez J-F]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:49:54 2008''