2w9d: Difference between revisions

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-{{Seed}}
-[[Image:2w9d.png|left|200px]]
-<!--
+==Structure of Fab fragment of the ICSM 18 - anti-Prp therapeutic antibody at 1.57 A resolution.==
-The line below this paragraph, containing "STRUCTURE_2w9d", creates the "Structure Box" on the page.
+<StructureSection load='2w9d' size='340' side='right'caption='[[2w9d]], [[Resolution|resolution]] 1.57&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2w9d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W9D FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-{{STRUCTURE_2w9d|  PDB=2w9d  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9d OCA], [https://pdbe.org/2w9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w9d RCSB], [https://www.ebi.ac.uk/pdbsum/2w9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9d ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9d_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w9d ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Prion infection is characterized by the conversion of host cellular prion protein (PrP(C)) into disease-related conformers (PrP(Sc)) and can be arrested in vivo by passive immunization with anti-PrP monoclonal antibodies. Here, we show that the ability of an antibody to cure prion-infected cells correlates with its binding affinity for PrP(C) rather than PrP(Sc). We have visualized this interaction at the molecular level by determining the crystal structure of human PrP bound to the Fab fragment of monoclonal antibody ICSM 18, which has the highest affinity for PrP(C) and the highest therapeutic potency in vitro and in vivo. In this crystal structure, human PrP is observed in its native PrP(C) conformation. Interactions between neighboring PrP molecules in the crystal structure are mediated by close homotypic contacts between residues at position 129 that lead to the formation of a 4-strand intermolecular beta-sheet. The importance of this residue in mediating protein-protein contact could explain the genetic susceptibility and prion strain selection determined by polymorphic residue 129 in human prion disease, one of the strongest common susceptibility polymorphisms known in any human disease.
-===STRUCTURE OF FAB FRAGMENT OF THE ICSM 18 - ANTI-PRP THERAPEUTIC ANTIBODY AT 1.57 A RESOLUTION.===
+Crystal structure of human prion protein bound to a therapeutic antibody.,Antonyuk SV, Trevitt CR, Strange RW, Jackson GS, Sangar D, Batchelor M, Cooper S, Fraser C, Jones S, Georgiou T, Khalili-Shirazi A, Clarke AR, Hasnain SS, Collinge J Proc Natl Acad Sci U S A. 2009 Feb 9. PMID:19204296<ref>PMID:19204296</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2w9d" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_19204296}}, adds the Publication Abstract to the page
+*[[Antibody 3D structures|Antibody 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 19204296 is the PubMed ID number.
+*[[Sandbox 20009|Sandbox 20009]]
--->
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-{{ABSTRACT_PUBMED_19204296}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-W9D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9D OCA].
+</StructureSection>
+[[Category: Large Structures]]
-==Reference==
-Crystal structure of human prion protein bound to a therapeutic antibody., Antonyuk SV, Trevitt CR, Strange RW, Jackson GS, Sangar D, Batchelor M, Cooper S, Fraser C, Jones S, Georgiou T, Khalili-Shirazi A, Clarke AR, Hasnain SS, Collinge J, Proc Natl Acad Sci U S A. 2009 Feb 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19204296 19204296]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: Antonyuk SV]]
-[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
+[[Category: Batchelor M]]
-[[Category: Fab]]
+[[Category: Clarke AR]]
-[[Category: Immune system]]
+[[Category: Collinge J]]
-[[Category: Prion]]
+[[Category: Cooper S]]
-[[Category: Prp]]
+[[Category: Fraser C]]
+[[Category: Georgiou T]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 20:03:20 2009''
+[[Category: Hasnain SS]]
+[[Category: Jackson GS]]
+[[Category: Jones S]]
+[[Category: Khalili-Shirazi A]]
+[[Category: Sangar D]]
+[[Category: Strange RW]]
+[[Category: Trevitt CR]]