2vul: Difference between revisions
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==Thermostable mutant of ENVIRONMENTALLY ISOLATED GH11 XYLANASE== | ==Thermostable mutant of ENVIRONMENTALLY ISOLATED GH11 XYLANASE== | ||
<StructureSection load='2vul' size='340' side='right' caption='[[2vul]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='2vul' size='340' side='right'caption='[[2vul]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vul]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2vul]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUL FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr><td class="sblockLbl"><b>[[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vul OCA], [https://pdbe.org/2vul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vul RCSB], [https://www.ebi.ac.uk/pdbsum/2vul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vul ProSAT]</span></td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </table> | ||
<table> | == Function == | ||
[https://www.uniprot.org/uniprot/B2LWN3_9BACT B2LWN3_9BACT] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vul_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vul_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vul ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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Engineering hyperthermostability into a GH11 xylanase is mediated by subtle changes to protein structure.,Dumon C, Varvak A, Wall MA, Flint JE, Lewis RJ, Lakey JH, Morland C, Luginbuhl P, Healey S, Todaro T, DeSantis G, Sun M, Parra-Gessert L, Tan X, Weiner DP, Gilbert HJ J Biol Chem. 2008 Aug 15;283(33):22557-64. Epub 2008 May 30. PMID:18515360<ref>PMID:18515360</ref> | Engineering hyperthermostability into a GH11 xylanase is mediated by subtle changes to protein structure.,Dumon C, Varvak A, Wall MA, Flint JE, Lewis RJ, Lakey JH, Morland C, Luginbuhl P, Healey S, Todaro T, DeSantis G, Sun M, Parra-Gessert L, Tan X, Weiner DP, Gilbert HJ J Biol Chem. 2008 Aug 15;283(33):22557-64. Epub 2008 May 30. PMID:18515360<ref>PMID:18515360</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2vul" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Desantis | [[Category: Desantis G]] | ||
[[Category: Dumon | [[Category: Dumon C]] | ||
[[Category: Flint | [[Category: Flint JE]] | ||
[[Category: Gilbert | [[Category: Gilbert HJ]] | ||
[[Category: Healey | [[Category: Healey S]] | ||
[[Category: Lakey | [[Category: Lakey JH]] | ||
[[Category: Lewis | [[Category: Lewis RJ]] | ||
[[Category: Luginbuhl | [[Category: Luginbuhl P]] | ||
[[Category: Parra-Gessert | [[Category: Parra-Gessert L]] | ||
[[Category: Sun | [[Category: Sun M]] | ||
[[Category: Tan | [[Category: Tan X]] | ||
[[Category: Todaro | [[Category: Todaro T]] | ||
[[Category: Varvak | [[Category: Varvak A]] | ||
[[Category: Wall | [[Category: Wall MA]] | ||
[[Category: Weiner | [[Category: Weiner DP]] | ||