2vjc: Difference between revisions

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-[[Image:2vjc.png|left|200px]]
-<!--
+==Torpedo Californica Acetylcholinesterase In Complex With A Non Hydrolysable Substrate Analogue, 4-Oxo-N,N,N- Trimethylpentanaminium - Orthorhombic space group - Dataset A at 150K==
-The line below this paragraph, containing "STRUCTURE_2vjc", creates the "Structure Box" on the page.
+<StructureSection load='2vjc' size='340' side='right'caption='[[2vjc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2vjc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetronarce_californica Tetronarce californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VJC FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCD:(4R)-4-HYDROXY-N,N,N-TRIMETHYLPENTAN-1-AMINIUM'>CCD</scene>, <scene name='pdbligand=CHH:N,N,N-TRIMETHYL-4-OXOPENTAN-1-AMINIUM'>CHH</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-{{STRUCTURE_2vjc|  PDB=2vjc  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vjc OCA], [https://pdbe.org/2vjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vjc RCSB], [https://www.ebi.ac.uk/pdbsum/2vjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vjc ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ACES_TETCF ACES_TETCF] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vj/2vjc_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vjc ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Although x-ray crystallography is the most widely used method for macromolecular structure determination, it does not provide dynamical information, and either experimental tricks or complementary experiments must be used to overcome the inherently static nature of crystallographic structures. Here we used specific x-ray damage during temperature-controlled crystallographic experiments at a third-generation synchrotron source to trigger and monitor (Shoot-and-Trap) structural changes putatively involved in an enzymatic reaction. In particular, a nonhydrolyzable substrate analogue of acetylcholinesterase, the "off-switch" at cholinergic synapses, was radiocleaved within the buried enzymatic active site. Subsequent product clearance, observed at 150 K but not at 100 K, indicated exit from the active site possibly via a "backdoor." The simple strategy described here is, in principle, applicable to any enzyme whose structure in complex with a substrate analogue is available and, therefore, could serve as a standard procedure in kinetic crystallography studies.
-===TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH A NON HYDROLYSABLE SUBSTRATE ANALOGUE, 4-OXO-N,N,N-TRIMETHYLPENTANAMINIUM - ORTHORHOMBIC SPACE GROUP -DATASET A AT 150K===
+Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography.,Colletier JP, Bourgeois D, Sanson B, Fournier D, Sussman JL, Silman I, Weik M Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11742-7. Epub 2008 Aug 13. PMID:18701720<ref>PMID:18701720</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_18701720}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2vjc" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 18701720 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_18701720}}
-==About this Structure==
-[[2vjc]] is a 2 chain structure of [[Acetylcholinesterase]] with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VJC OCA].
 ==See Also==
-*[[Acetylcholinesterase]]
+*[[Acetylcholinesterase 3D structures|Acetylcholinesterase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:18701720</ref><references group="xtra"/>
+__TOC__
-[[Category: Acetylcholinesterase]]
+</StructureSection>
-[[Category: Torpedo californica]]
+[[Category: Large Structures]]
-[[Category: Bourgeois, D.]]
+[[Category: Tetronarce californica]]
-[[Category: Colletier, J P.]]
+[[Category: Bourgeois D]]
-[[Category: Fournier, D.]]
+[[Category: Colletier JP]]
-[[Category: Silman, I.]]
+[[Category: Fournier D]]
-[[Category: Sussman, J L.]]
+[[Category: Silman I]]
-[[Category: Weik, M.]]
+[[Category: Sussman JL]]
-[[Category: Alternative splicing]]
+[[Category: Weik M]]
-[[Category: Cell junction]]
-[[Category: Glycoprotein]]
-[[Category: Gpi-anchor]]
-[[Category: Hydrolase]]
-[[Category: Kinetic crystallography]]
-[[Category: Lipoprotein]]
-[[Category: Membrane]]
-[[Category: Neurotransmitter degradation]]
-[[Category: Serine esterase]]
-[[Category: Structural dynamic]]
-[[Category: Substrate analogue]]
-[[Category: Synapse]]
-[[Category: Xray damage]]