2rpv: Difference between revisions

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-[[Image:2rpv.png|left|200px]]
-{{STRUCTURE_2rpv|  PDB=2rpv  |  SCENE=  }}
+==Solution Structure of GB1 with LBT probe==
+<StructureSection load='2rpv' size='340' side='right'caption='[[2rpv]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2rpv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RPV FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rpv OCA], [https://pdbe.org/2rpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rpv RCSB], [https://www.ebi.ac.uk/pdbsum/2rpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rpv ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rp/2rpv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rpv ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudo-contact shifts and residual dipolar couplings. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag. Here we report a lanthanide-binding peptide tag anchored via two points to the target proteins. Compared to conventional single-point attached tags, the two-point linked tag provides two to threefold stronger anisotropic effects. Though there is slight residual mobility of the lanthanide-binding tag, the present tag provides a higher anisotropic paramagnetic effect.
-===Solution Structure of GB1 with LBT probe===
+Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.,Saio T, Ogura K, Yokochi M, Kobashigawa Y, Inagaki F J Biomol NMR. 2009 Jul;44(3):157-66. Epub 2009 May 26. PMID:19468839<ref>PMID:19468839</ref>
-{{ABSTRACT_PUBMED_19468839}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2rpv" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[2rpv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._'group_g' Streptococcus sp. 'group g']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RPV OCA].
+*[[Protein G|Protein G]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019468839</ref><references group="xtra"/>
+__TOC__
-[[Category: Streptococcus sp. 'group g']]
+</StructureSection>
-[[Category: Inagaki, F.]]
+[[Category: Large Structures]]
-[[Category: Kobashigawa, Y.]]
+[[Category: Streptococcus sp. 'group G']]
-[[Category: Ogura, K.]]
+[[Category: Inagaki F]]
-[[Category: Saio, T.]]
+[[Category: Kobashigawa Y]]
-[[Category: Yokochi, M.]]
+[[Category: Ogura K]]
-[[Category: Cell wall]]
+[[Category: Saio T]]
-[[Category: Gb1]]
+[[Category: Yokochi M]]
-[[Category: Igg-binding protein]]
-[[Category: Immune system]]
-[[Category: Lanthanide binding peptide]]
-[[Category: Lbt]]
-[[Category: Olivia]]
-[[Category: Paramagnetic effect]]
-[[Category: Peptidoglycan-anchor]]
-[[Category: Secreted]]