2ra5: Difference between revisions

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New page: left|200px<br /><applet load="2ra5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ra5, resolution 2.40Å" /> '''Crystal structure of...
 
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[[Image:2ra5.jpg|left|200px]]<br /><applet load="2ra5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ra5, resolution 2.40&Aring;" />
'''Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor'''<br />


==Overview==
==Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor==
We tested the general applicability of in situ proteolysis to form protein, crystals suitable for structure determination by adding a protease, (chymotrypsin or trypsin) digestion step to crystallization trials of 55, bacterial and 14 human proteins that had proven recalcitrant to our best, efforts at crystallization or structure determination. This is a work in, progress; so far we determined structures of 9 bacterial proteins and the, human aminoimidazole ribonucleotide synthetase (AIRS) domain.
<StructureSection load='2ra5' size='340' side='right'caption='[[2ra5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2ra5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RA5 FirstGlance]. <br>
2RA5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with <scene name='pdbligand=SRT:'>SRT</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA5 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ra5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra5 OCA], [https://pdbe.org/2ra5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ra5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ra5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ra5 ProSAT], [https://www.topsan.org/Proteins/MCSG/2ra5 TOPSAN]</span></td></tr>
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Leo RD, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Nov 4;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17982461 17982461]
</table>
[[Category: Bacteria]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q9RKT6_STRCO Q9RKT6_STRCO]  
[[Category: Edwards, A.M.]]
== Evolutionary Conservation ==
[[Category: Joachimiak, A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kim, Y.]]
Check<jmol>
[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]
  <jmolCheckbox>
[[Category: Savchenko, A.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2ra5_consurf.spt"</scriptWhenChecked>
[[Category: Xu, X.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
[[Category: Zheng, H.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: IPA]]
  </jmolCheckbox>
[[Category: SRT]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ra5 ConSurf].
[[Category: beta structure]]
<div style="clear:both"></div>
[[Category: dna-binding]]
__TOC__
[[Category: mcsg]]
</StructureSection>
[[Category: midwest center for structural genomics]]
[[Category: Large Structures]]
[[Category: protein structure initiative]]
[[Category: Edwards AM]]
[[Category: psi-2]]
[[Category: Joachimiak A]]
[[Category: structural genomics]]
[[Category: Kim Y]]
[[Category: transcription]]
[[Category: Savchenko A]]
[[Category: transcription regulation]]
[[Category: Xu X]]
[[Category: utra domain]]
[[Category: Zheng H]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:07:11 2008''

Latest revision as of 08:29, 17 October 2024

Crystal structure of the putative transcriptional regulator from Streptomyces coelicolorCrystal structure of the putative transcriptional regulator from Streptomyces coelicolor

Structural highlights

2ra5 is a 1 chain structure with sequence from Streptomyces coelicolor A3(2). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

Q9RKT6_STRCO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2ra5, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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