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==Crystal Structure and Functional Study of the Bowman-Birk Inhibitor from Rice Bran in Complex with Bovine Trypsin==
==Crystal Structure and Functional Study of the Bowman-Birk Inhibitor from Rice Bran in Complex with Bovine Trypsin==
<StructureSection load='2qn5' size='340' side='right' caption='[[2qn5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
<StructureSection load='2qn5' size='340' side='right'caption='[[2qn5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2qn5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QN5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2qn5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QN5 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1c2a|1c2a]], [[1tx6|1tx6]], [[1k9b|1k9b]], [[1s0q|1s0q]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qn5 OCA], [https://pdbe.org/2qn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qn5 RCSB], [https://www.ebi.ac.uk/pdbsum/2qn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qn5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qn5 OCA], [http://pdbe.org/2qn5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qn5 RCSB], [http://www.ebi.ac.uk/pdbsum/2qn5 PDBsum]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IBBR_ORYSJ IBBR_ORYSJ]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/2qn5_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/2qn5_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qn5 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qn5 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bowman-Birk inhibitors (BBIs) are cysteine-rich proteins with inhibitory activity against proteases that are widely distributed in monocot and dicot species. The expression of rice BBI from Oryza sativa is up-regulated and induced by pathogens or insects during germination of rice seeds. The rice BBI (RBTI) of molecular weight 15 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of rice BBI crystals at a resolution of 2.07 A, the unit cell belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.37, b = 96.69, c = 100.36 A. Preliminary analysis indicates four BBI molecules in an asymmetric unit, with a solvent content of 58.29%.
Purification, crystallization and preliminary X-ray crystallographic analysis of rice Bowman-Birk inhibitor from Oryza sativa.,Lin YH, Li HT, Huang YC, Hsieh YC, Guan HH, Liu MY, Chang T, Wang AH, Chen CJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt, 6):522-4. Epub 2006 May 5. PMID:16754971<ref>PMID:16754971</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2qn5" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Trypsin|Trypsin]]
*[[Trypsin 3D structures|Trypsin 3D structures]]
*[[Trypsin inhibitor|Trypsin inhibitor]]
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Oryza sativa]]
[[Category: Large Structures]]
[[Category: Trypsin]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Chen, C J]]
[[Category: Chen CJ]]
[[Category: Guan, H H]]
[[Category: Guan HH]]
[[Category: Hsieh, Y C]]
[[Category: Hsieh YC]]
[[Category: Li, H T]]
[[Category: Li HT]]
[[Category: Lin, Y H]]
[[Category: Lin YH]]
[[Category: Wang, A H.J]]
[[Category: Wang AHJ]]
[[Category: Bowman-birk inhibitor]]
[[Category: Digestion]]
[[Category: Hetero-dimer]]
[[Category: Hydrolase]]
[[Category: Hydrolase inhibitor-hydrolase complex]]
[[Category: Metal-binding]]
[[Category: Monocotyledonous plant]]
[[Category: Plant-pest system]]
[[Category: Protease inhibitor]]
[[Category: Protease-inhibitor]]
[[Category: Rbti]]
[[Category: Reactive-site loop]]
[[Category: Secreted]]
[[Category: Serine protease inhibitor]]
[[Category: Zymogen]]

Latest revision as of 08:28, 17 October 2024

Crystal Structure and Functional Study of the Bowman-Birk Inhibitor from Rice Bran in Complex with Bovine TrypsinCrystal Structure and Functional Study of the Bowman-Birk Inhibitor from Rice Bran in Complex with Bovine Trypsin

Structural highlights

2qn5 is a 2 chain structure with sequence from Bos taurus and Oryza sativa Japonica Group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IBBR_ORYSJ

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2qn5, resolution 3.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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