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[[Image:2ph4.jpg|left|200px]]


{{Structure
==Crystal structure of a novel Arg49 phospholipase A2 homologue from Zhaoermia mangshanensis venom==
|PDB= 2ph4 |SIZE=350|CAPTION= <scene name='initialview01'>2ph4</scene>, resolution 2.05&Aring;
<StructureSection load='2ph4' size='340' side='right'caption='[[2ph4]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+301'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+302'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+B+303'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+B+304'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+B+305'>AC5</scene>, <scene name='pdbsite=AC6:Peg+Binding+Site+For+Residue+A+401'>AC6</scene> and <scene name='pdbsite=AC7:Peg+Binding+Site+For+Residue+B+402'>AC7</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>
<table><tr><td colspan='2'>[[2ph4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Protobothrops_mangshanensis Protobothrops mangshanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PH4 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ph4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ph4 OCA], [https://pdbe.org/2ph4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ph4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ph4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ph4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA2H_PROMB PA2H_PROMB] Snake venom phospholipase A2 homolog that induces myonecrosis, and edema. Has low myotoxic activity.<ref>PMID:16635500</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ph/2ph4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ph4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The venom of Zhaoermia mangshanensis, encountered solely in Mt Mang in China's Hunan Province, exhibits coagulant, phosphodiesterase, l-amino acid oxidase, kallikrein, phospholipase A2 and myotoxic activities. The catalytically inactive PLA2 homolog referred to as zhaoermiatoxin is highly myotoxic and displays high myonecrotic and edema activities. Zhaoermiatoxin possesses a molecular weight of 13,972Da, consists of 121 amino-acid residues cross-linked by seven disulfide bridges and shares high sequence homology with Lys49-PLA2s from the distantly related Asian pitvipers. However, zhaoermiatoxin possesses an arginine residue at position 49 instead of a lysine, thereby suggesting a secondary Lys49--&gt;Arg substitution which results in a catalytically inactive protein. We have determined the first crystal structure of zhaoermiatoxin, an Arg49-PLA2, from Zhaoermia mangshanensis venom at 2.05 angstroms resolution, which represents a novel member of phospholipase A2 family. In this structure, unlike the Lys49 PLA2s, the C-terminus is well ordered and an unexpected non-polarized state of the putative calcium-binding loop due to the flip of Lys122 towards the bulk solvent is observed. The orientation of the Arg-49 side chain results in a similar binding mode to that observed in the Lys49 PLA2s; however, the guadinidium group is tri-coordinated by carbonyl oxygen atoms of the putative calcium-binding loop, whereas the Nzeta atom of lysine is tetra-coordinated as a result of the different conformation adopted by the putative calcium-binding loop.


'''Crystal structure of a novel Arg49 phospholipase A2 homologue from Zhaoermia mangshanensis venom'''
Crystal structure of a novel myotoxic Arg49 phospholipase A2 homolog (zhaoermiatoxin) from Zhaoermia mangshanensis snake venom: insights into Arg49 coordination and the role of Lys122 in the polarization of the C-terminus.,Murakami MT, Kuch U, Betzel C, Mebs D, Arni RK Toxicon. 2008 Apr;51(5):723-35. Epub 2007 Nov 29. PMID:18295812<ref>PMID:18295812</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ph4" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The venom of Zhaoermia mangshanensis, encountered solely in Mt Mang in China's Hunan Province, exhibits coagulant, phosphodiesterase, l-amino acid oxidase, kallikrein, phospholipase A(2) and myotoxic activities. The catalytically inactive PLA(2) homolog referred to as zhaoermiatoxin is highly myotoxic and displays high myonecrotic and edema activities. Zhaoermiatoxin possesses a molecular weight of 13,972Da, consists of 121 amino-acid residues cross-linked by seven disulfide bridges and shares high sequence homology with Lys49-PLA(2)s from the distantly related Asian pitvipers. However, zhaoermiatoxin possesses an arginine residue at position 49 instead of a lysine, thereby suggesting a secondary Lys49--&gt;Arg substitution which results in a catalytically inactive protein. We have determined the first crystal structure of zhaoermiatoxin, an Arg49-PLA(2), from Zhaoermia mangshanensis venom at 2.05A resolution, which represents a novel member of phospholipase A(2) family. In this structure, unlike the Lys49 PLA(2)s, the C-terminus is well ordered and an unexpected non-polarized state of the putative calcium-binding loop due to the flip of Lys122 towards the bulk solvent is observed. The orientation of the Arg-49 side chain results in a similar binding mode to that observed in the Lys49 PLA(2)s; however, the guadinidium group is tri-coordinated by carbonyl oxygen atoms of the putative calcium-binding loop, whereas the Nzeta atom of lysine is tetra-coordinated as a result of the different conformation adopted by the putative calcium-binding loop.
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
 
*[[Phospholipase A2 homolog|Phospholipase A2 homolog]]
==About this Structure==
== References ==
2PH4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zhaoermia_mangshanensis Zhaoermia mangshanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PH4 OCA].
<references/>
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of a novel myotoxic Arg49 phospholipase A(2) homolog (zhaoermiatoxin) from Zhaoermia mangshanensis snake venom: Insights into Arg49 coordination and the role of Lys122 in the polarization of the C-terminus., Murakami MT, Kuch U, Betzel C, Mebs D, Arni RK, Toxicon. 2008 Apr;51(5):723-35. Epub 2007 Nov 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18295812 18295812]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Protobothrops mangshanensis]]
[[Category: Zhaoermia mangshanensis]]
[[Category: Arni RK]]
[[Category: Arni, R K.]]
[[Category: Kuch U]]
[[Category: Kuch, U.]]
[[Category: Mebs D]]
[[Category: Mebs, D.]]
[[Category: Murakami MT]]
[[Category: Murakami, M T.]]
[[Category: PEG]]
[[Category: SO4]]
[[Category: arg49]]
[[Category: myotoxin]]
[[Category: phospholipase a2]]
[[Category: snake venom]]
 
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