2osh: Difference between revisions

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-{{Seed}}
-[[Image:2osh.png|left|200px]]
-<!--
+==crystal structure of Natratoxin, a snake sPLA2 that blocks A-type K+ channel==
-The line below this paragraph, containing "STRUCTURE_2osh", creates the "Structure Box" on the page.
+<StructureSection load='2osh' size='340' side='right'caption='[[2osh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2osh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OSH FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2osh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2osh OCA], [https://pdbe.org/2osh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2osh RCSB], [https://www.ebi.ac.uk/pdbsum/2osh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2osh ProSAT]</span></td></tr>
-{{STRUCTURE_2osh|  PDB=2osh  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PA2A_NAJAT PA2A_NAJAT]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/2osh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2osh ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Snake secreted phospholipasesA2 (sPLA2s) are widely used as pharmacological tools to investigate their role in diverse pathophysiological processes. Some members of snake venom sPLA2s have been found to block voltage-activated K(+) channels (K(v) channels). However, most studies involved in their effects on ion channels were indirectly performed on motor nerve terminals while few studies were directly done on native neurons. Here, a novel snake sPLA2 peptide neurotoxin, Natratoxin, composed of 119 amino acid residues and purified from Naja atra venom was reported. It was characterized using whole-cell patch-clamp in acutely dissociated rat dorsal root ganglion (DRG) neurons. It was found to effectively inhibit A-type K(+) currents and cause alterations of channel gating characters, such as the shifts of steady-state activation and inactivation curves to hyperpolarization direction and changes of V(1/2) and slope factor. Therefore, Natratoxin was suggested to be a gating modifier of K(v) channel. In addition, this inhibitory effect was found to be independent of its enzymatic activity. These results suggested that the toxin enacted its inhibitory effect by binding to K(v) channel. To further elucidate the structural basis for this electrophysiological phenomenon, we determined the crystal structure of Natratoxin at 2.2 A resolution by molecular replacement method and refined to an R-factor of 0.190. The observed overall fold has a different structural organization from other K(+) channel inhibitors in animal toxins. Compared with other K(v) channel inhibitors, a similar putative functional surface in its C-terminal was revealed to contribute to protein-protein interaction in such a blocking effect. Our results demonstrated that the spatial distribution of key amino acid residues matters most in the recognition of this toxin towards its channel target rather than its type of fold.
-===crystal structure of Natratoxin, a snake sPLA2 that blocks A-type K+ channel===
+Crystal structure of Natratoxin, a novel snake secreted phospholipaseA2 neurotoxin from Naja atra venom inhibiting A-type K+ currents.,Hu P, Sun L, Zhu ZQ, Hou XW, Wang S, Yu SS, Wang HL, Zhang P, Wang M, Niu LW, Teng MK, Ruan DY Proteins. 2008 Aug;72(2):673-83. PMID:18247353<ref>PMID:18247353</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2osh" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18247353}}, adds the Publication Abstract to the page
+*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18247353 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18247353}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-OSH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OSH OCA].
-==Reference==
-Crystal structure of Natratoxin, a novel snake secreted phospholipaseA2 neurotoxin from Naja atra venom inhibiting A-type K+ currents., Hu P, Sun L, Zhu ZQ, Hou XW, Wang S, Yu SS, Wang HL, Zhang P, Wang M, Niu LW, Teng MK, Ruan DY, Proteins. 2008 Aug;72(2):673-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18247353 18247353]
 [[Category: Naja atra]]
-[[Category: Single protein]]
+[[Category: Sun L]]
-[[Category: Sun, L.]]
+[[Category: Teng MK]]
-[[Category: Teng, M K.]]
-[[Category: A-type k+ channel inhibitor]]
-[[Category: Hydrolase]]
-[[Category: Naja atra venom]]
-[[Category: Phospholipase a2]]
-[[Category: Structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov  5 10:40:19 2008''