2iwt: Difference between revisions

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-[[Image:2iwt.jpg|left|200px]]
- {{Structure
+==Thioredoxin h2 (HvTrxh2) in a mixed disulfide complex with the target protein BASI==
-|PDB= 2iwt |SIZE=350|CAPTION= <scene name='initialview01'>2iwt</scene>, resolution 2.30&Aring;
+<StructureSection load='2iwt' size='340' side='right'caption='[[2iwt]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-|SITE= <scene name='pdbsite=AC1:Flc+Binding+Site+For+Chain+B'>AC1</scene>
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=FLC:CITRATE ANION'>FLC</scene>
+<table><tr><td colspan='2'>[[2iwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IWT FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwt OCA], [https://pdbe.org/2iwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iwt RCSB], [https://www.ebi.ac.uk/pdbsum/2iwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwt ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IAAS_HORVU IAAS_HORVU] This protein inhibits independently subtilisin and alpha-amylase.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iwt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iwt ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Thioredoxin is ubiquitous and regulates various target proteins through disulfide bond reduction. We report the structure of thioredoxin (HvTrxh2 from barley) in a reaction intermediate complex with a protein substrate, barley alpha-amylase/subtilisin inhibitor (BASI). The crystal structure of this mixed disulfide shows a conserved hydrophobic motif in thioredoxin interacting with a sequence of residues from BASI through van der Waals contacts and backbone-backbone hydrogen bonds. The observed structural complementarity suggests that the recognition of features around protein disulfides plays a major role in the specificity and protein disulfide reductase activity of thioredoxin. This novel insight into the function of thioredoxin constitutes a basis for comprehensive understanding of its biological role. Moreover, comparison with structurally related proteins shows that thioredoxin shares a mechanism with glutaredoxin and glutathione transferase for correctly positioning substrate cysteine residues at the catalytic groups but possesses a unique structural element that allows recognition of protein disulfides.
-'''THIOREDOXIN H2 (HVTRXH2) IN A MIXED DISULFIDE COMPLEX WITH THE TARGET PROTEIN BASI'''
+Structural basis for target protein recognition by the protein disulfide reductase thioredoxin.,Maeda K, Hagglund P, Finnie C, Svensson B, Henriksen A Structure. 2006 Nov;14(11):1701-10. PMID:17098195<ref>PMID:17098195</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2iwt" style="background-color:#fffaf0;"></div>
-==Overview==
+==See Also==
-Thioredoxin is ubiquitous and regulates various target proteins through disulfide bond reduction. We report the structure of thioredoxin (HvTrxh2 from barley) in a reaction intermediate complex with a protein substrate, barley alpha-amylase/subtilisin inhibitor (BASI). The crystal structure of this mixed disulfide shows a conserved hydrophobic motif in thioredoxin interacting with a sequence of residues from BASI through van der Waals contacts and backbone-backbone hydrogen bonds. The observed structural complementarity suggests that the recognition of features around protein disulfides plays a major role in the specificity and protein disulfide reductase activity of thioredoxin. This novel insight into the function of thioredoxin constitutes a basis for comprehensive understanding of its biological role. Moreover, comparison with structurally related proteins shows that thioredoxin shares a mechanism with glutaredoxin and glutathione transferase for correctly positioning substrate cysteine residues at the catalytic groups but possesses a unique structural element that allows recognition of protein disulfides.
+*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-IWT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA].
+__TOC__
+</StructureSection>
-==Reference==
-Structural basis for target protein recognition by the protein disulfide reductase thioredoxin., Maeda K, Hagglund P, Finnie C, Svensson B, Henriksen A, Structure. 2006 Nov;14(11):1701-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098195 17098195]
 [[Category: Hordeum vulgare]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Finnie, C.]]
+[[Category: Finnie C]]
-[[Category: Hagglund, P.]]
+[[Category: Hagglund P]]
-[[Category: Henriksen, A.]]
+[[Category: Henriksen A]]
-[[Category: Maeda, K.]]
+[[Category: Maeda K]]
-[[Category: Svensson, B.]]
+[[Category: Svensson B]]
-[[Category: FLC]]
-[[Category: alpha-amylase inhibitor]]
-[[Category: amy2]]
-[[Category: basi]]
-[[Category: disulfide intermediate]]
-[[Category: disulfide reductase]]
-[[Category: oxidoreductase]]
-[[Category: protease inhibitor]]
-[[Category: redox]]
-[[Category: serine protease inhibitor]]
-[[Category: substrate recognition]]
-[[Category: thioredoxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:34:25 2008''