2iwt: Difference between revisions

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-[[Image:2iwt.png|left|200px]]
-{{STRUCTURE_2iwt|  PDB=2iwt  |  SCENE=  }}
+==Thioredoxin h2 (HvTrxh2) in a mixed disulfide complex with the target protein BASI==
+<StructureSection load='2iwt' size='340' side='right'caption='[[2iwt]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2iwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IWT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwt OCA], [https://pdbe.org/2iwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iwt RCSB], [https://www.ebi.ac.uk/pdbsum/2iwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwt ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IAAS_HORVU IAAS_HORVU] This protein inhibits independently subtilisin and alpha-amylase.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iwt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iwt ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Thioredoxin is ubiquitous and regulates various target proteins through disulfide bond reduction. We report the structure of thioredoxin (HvTrxh2 from barley) in a reaction intermediate complex with a protein substrate, barley alpha-amylase/subtilisin inhibitor (BASI). The crystal structure of this mixed disulfide shows a conserved hydrophobic motif in thioredoxin interacting with a sequence of residues from BASI through van der Waals contacts and backbone-backbone hydrogen bonds. The observed structural complementarity suggests that the recognition of features around protein disulfides plays a major role in the specificity and protein disulfide reductase activity of thioredoxin. This novel insight into the function of thioredoxin constitutes a basis for comprehensive understanding of its biological role. Moreover, comparison with structurally related proteins shows that thioredoxin shares a mechanism with glutaredoxin and glutathione transferase for correctly positioning substrate cysteine residues at the catalytic groups but possesses a unique structural element that allows recognition of protein disulfides.
-===THIOREDOXIN H2 (HVTRXH2) IN A MIXED DISULFIDE COMPLEX WITH THE TARGET PROTEIN BASI===
+Structural basis for target protein recognition by the protein disulfide reductase thioredoxin.,Maeda K, Hagglund P, Finnie C, Svensson B, Henriksen A Structure. 2006 Nov;14(11):1701-10. PMID:17098195<ref>PMID:17098195</ref>
-{{ABSTRACT_PUBMED_17098195}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2iwt" style="background-color:#fffaf0;"></div>
-[[2iwt]] is a 2 chain structure of [[Thioredoxin]] with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA].
 ==See Also==
-*[[Thioredoxin|Thioredoxin]]
+*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:017098195</ref><ref group="xtra">PMID:015765494</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Hordeum vulgare]]
-[[Category: Finnie, C.]]
+[[Category: Large Structures]]
-[[Category: Hagglund, P.]]
+[[Category: Finnie C]]
-[[Category: Henriksen, A.]]
+[[Category: Hagglund P]]
-[[Category: Maeda, K.]]
+[[Category: Henriksen A]]
-[[Category: Svensson, B.]]
+[[Category: Maeda K]]
-[[Category: Alpha-amylase inhibitor]]
+[[Category: Svensson B]]
-[[Category: Amy2]]
-[[Category: Basi]]
-[[Category: Disulfide intermediate]]
-[[Category: Disulfide reductase]]
-[[Category: Oxidoreductase]]
-[[Category: Protease inhibitor]]
-[[Category: Redox]]
-[[Category: Serine protease inhibitor]]
-[[Category: Substrate recognition]]
-[[Category: Thioredoxin]]