2h5f: Difference between revisions

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-{{Seed}}
-[[Image:2h5f.png|left|200px]]
-<!--
+==Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity==
-The line below this paragraph, containing "STRUCTURE_2h5f", creates the "Structure Box" on the page.
+<StructureSection load='2h5f' size='340' side='right'caption='[[2h5f]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2h5f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Boiga_dendrophila Boiga dendrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H5F FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-{{STRUCTURE_2h5f|  PDB=2h5f  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h5f OCA], [https://pdbe.org/2h5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h5f RCSB], [https://www.ebi.ac.uk/pdbsum/2h5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h5f ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/3NB_BOIDE 3NB_BOIDE] This bird-specific postsynaptic neurotoxin irreversibly binds and inhibits the chick muscle alpha-1-beta-1-gamma-delta (CHRNA1-CHRNB1-CHRNG-CHNRD) nicotinic acetylcholine receptor (nAChR) 100-fold more compared with the mouse receptor. The weak binding to mouse receptor is reversible.<ref>PMID:16864572</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/2h5f_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h5f ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Boiga dendrophila (mangrove catsnake) is a colubrid snake that lives in Southeast Asian lowland rainforests and mangrove swamps and that preys primarily on birds. We have isolated, purified, and sequenced a novel toxin from its venom, which we named denmotoxin. It is a monomeric polypeptide of 77 amino acid residues with five disulfide bridges. In organ bath experiments, it displayed potent postsynaptic neuromuscular activity and irreversibly inhibited indirectly stimulated twitches in chick biventer cervicis nerve-muscle preparations. In contrast, it induced much smaller and readily reversible inhibition of electrically induced twitches in mouse hemidiaphragm nerve-muscle preparations. More precisely, the chick muscle alpha(1)betagammadelta-nicotinic acetylcholine receptor was 100-fold more susceptible compared with the mouse receptor. These data indicate that denmotoxin has a bird-specific postsynaptic activity. We chemically synthesized denmotoxin, crystallized it, and solved its crystal structure at 1.9 A by the molecular replacement method. The toxin structure adopts a non-conventional three-finger fold with an additional (fifth) disulfide bond in the first loop and seven additional residues at its N terminus, which is blocked by a pyroglutamic acid residue. This is the first crystal structure of a three-finger toxin from colubrid snake venom and the first fully characterized bird-specific toxin. Denmotoxin illustrates the relationship between toxin specificity and the primary prey type that constitutes the snake's diet.
-===Denmotoxin: A the three-finger toxin from colubrid snake Boiga dendrophila with bird-specific activity===
+Denmotoxin, a three-finger toxin from the colubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity.,Pawlak J, Mackessy SP, Fry BG, Bhatia M, Mourier G, Fruchart-Gaillard C, Servent D, Menez R, Stura E, Menez A, Kini RM J Biol Chem. 2006 Sep 29;281(39):29030-41. Epub 2006 Jul 24. PMID:16864572<ref>PMID:16864572</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_16864572}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2h5f" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 16864572 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16864572}}
+__TOC__
+</StructureSection>
-==About this Structure==
-H5F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Boiga_dendrophila Boiga dendrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5F OCA].
-==Reference==
-Denmotoxin, a three-finger toxin from the colubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity., Pawlak J, Mackessy SP, Fry BG, Bhatia M, Mourier G, Fruchart-Gaillard C, Servent D, Menez R, Stura E, Menez A, Kini RM, J Biol Chem. 2006 Sep 29;281(39):29030-41. Epub 2006 Jul 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16864572 16864572]
 [[Category: Boiga dendrophila]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Kini, R M.]]
+[[Category: Kini RM]]
-[[Category: Pawlak, J.]]
+[[Category: Pawlak J]]
-[[Category: Stura, E A.]]
+[[Category: Stura EA]]
-[[Category: Neurotoxin]]
-[[Category: Snake venom]]
-[[Category: Three-finger toxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:12:39 2008''