2c9i: Difference between revisions

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-[[Image:2c9i.png|left|200px]]
-{{STRUCTURE_2c9i|  PDB=2c9i  |  SCENE=  }}
+==Structure of the fluorescent protein asFP499 from Anemonia sulcata==
+<StructureSection load='2c9i' size='340' side='right'caption='[[2c9i]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2c9i]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Anemonia_sulcata Anemonia sulcata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C9I FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c9i OCA], [https://pdbe.org/2c9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c9i RCSB], [https://www.ebi.ac.uk/pdbsum/2c9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c9i ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9GPI6_ANESU Q9GPI6_ANESU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/2c9i_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c9i ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Despite their similar fold topologies, anthozoan fluorescent proteins (FPs) can exhibit widely different optical properties, arising either from chemical modification of the chromophore itself or from specific interactions of the chromophore with the surrounding protein moiety. Here we present a structural and spectroscopic investigation of the green FP asFP499 from the sea anemone Anemonia sulcata var. rufescens to explore the effects of the protein environment on the chromophore. The optical absorption and fluorescence spectra reveal two discrete species populated in significant proportions over a wide pH range. Moreover, multiple protonation reactions are evident from the observed pH-dependent spectral changes. The x-ray structure of asFP499, determined by molecular replacement at a resolution of 1.85 A, shows the typical beta-barrel fold of the green FP from Aequorea victoria (avGFP). In its center, the chromophore, formed from the tripeptide Gln(63)-Tyr(64)-Gly(65), is tightly held by multiple hydrogen bonds in a polar cage that is structurally quite dissimilar to that of avGFP. The x-ray structure provides interesting clues as to how the spectroscopic properties are fine tuned by the chromophore environment.
-===STRUCTURE OF THE FLUORESCENT PROTEIN ASFP499 FROM ANEMONIA SULCATA===
+Chromophore-protein interactions in the anthozoan green fluorescent protein asFP499.,Nienhaus K, Renzi F, Vallone B, Wiedenmann J, Nienhaus GU Biophys J. 2006 Dec 1;91(11):4210-20. Epub 2006 Sep 15. PMID:16980366<ref>PMID:16980366</ref>
-{{ABSTRACT_PUBMED_16980366}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2c9i" style="background-color:#fffaf0;"></div>
-[[2c9i]] is a 8 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Anemonia_sulcata Anemonia sulcata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9I OCA].
 ==See Also==
-*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
+== References ==
-*[[Green Fluorescent Protein|Green Fluorescent Protein]]
+<references/>
-*[[Sandbox104|Sandbox104]]
+__TOC__
-*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:016980366</ref><references group="xtra"/>
 [[Category: Anemonia sulcata]]
-[[Category: Nienhaus, G U.]]
+[[Category: Large Structures]]
-[[Category: Nienhaus, K.]]
+[[Category: Nienhaus GU]]
-[[Category: Renzi, F.]]
+[[Category: Nienhaus K]]
-[[Category: Vallone, B.]]
+[[Category: Renzi F]]
-[[Category: Wiedenmann, J.]]
+[[Category: Vallone B]]
-[[Category: Beta-barrel]]
+[[Category: Wiedenmann J]]
-[[Category: Bioluminescence]]
-[[Category: Fluorescent protein]]
-[[Category: Luminescence]]
-[[Category: Luminescent protein]]