2bf1: Difference between revisions

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OCA

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-[[Image:2bf1.gif|left|200px]]<br /><applet load="2bf1" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2bf1, resolution 4.00&Aring;" />
-'''STRUCTURE OF AN UNLIGANDED AND FULLY-GLYCOSYLATED SIV GP120 ENVELOPE GLYCOPROTEIN'''<br />
-==Overview==
+==Structure of an unliganded and fully-glycosylated SIV gp120 envelope glycoprotein==
-Envelope glycoproteins of human and simian immunodeficiency virus (HIV and, SIV) undergo a series of conformational changes when they interact with, receptor (CD4) and co-receptor on the surface of a potential host cell, leading ultimately to fusion of viral and cellular membranes. Structures, of fragments of gp120 and gp41 from the envelope protein are known, in, conformations corresponding to their post-attachment and postfusion, states, respectively. We report the crystal structure, at 4 A resolution, of a fully glycosylated SIV gp120 core, in a conformation representing its, prefusion state, before interaction with CD4. Parts of the protein have a, markedly different organization than they do in the CD4-bound state., Comparison of the unliganded and CD4-bound structures leads to a model for, events that accompany receptor engagement of an envelope glycoprotein, trimer. The two conformations of gp120 also present distinct antigenic, surfaces. We identify the binding site for a compound that inhibits viral, entry.
+<StructureSection load='2bf1' size='340' side='right'caption='[[2bf1]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2bf1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BF1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf1 OCA], [https://pdbe.org/2bf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bf1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bf1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q07374_SIV Q07374_SIV]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bf1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bf1 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Envelope glycoproteins of human and simian immunodeficiency virus (HIV and SIV) undergo a series of conformational changes when they interact with receptor (CD4) and co-receptor on the surface of a potential host cell, leading ultimately to fusion of viral and cellular membranes. Structures of fragments of gp120 and gp41 from the envelope protein are known, in conformations corresponding to their post-attachment and postfusion states, respectively. We report the crystal structure, at 4 A resolution, of a fully glycosylated SIV gp120 core, in a conformation representing its prefusion state, before interaction with CD4. Parts of the protein have a markedly different organization than they do in the CD4-bound state. Comparison of the unliganded and CD4-bound structures leads to a model for events that accompany receptor engagement of an envelope glycoprotein trimer. The two conformations of gp120 also present distinct antigenic surfaces. We identify the binding site for a compound that inhibits viral entry.
-==About this Structure==
+Structure of an unliganded simian immunodeficiency virus gp120 core.,Chen B, Vogan EM, Gong H, Skehel JJ, Wiley DC, Harrison SC Nature. 2005 Feb 24;433(7028):834-41. PMID:15729334<ref>PMID:15729334</ref>
-BF1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus] with NAG and MAN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BF1 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structure of an unliganded simian immunodeficiency virus gp120 core., Chen B, Vogan EM, Gong H, Skehel JJ, Wiley DC, Harrison SC, Nature. 2005 Feb 24;433(7028):834-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15729334 15729334]
+</div>
+<div class="pdbe-citations 2bf1" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Gp120 3D structures|Gp120 3D structures]]
+*[[Immunodeficiency virus protease 3D structures|Immunodeficiency virus protease 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Simian immunodeficiency virus]]
-[[Category: Single protein]]
+[[Category: Chen B]]
-[[Category: Chen, B.]]
+[[Category: Gong H]]
-[[Category: Gong, H.]]
+[[Category: Harrison SC]]
-[[Category: Harrison, S.C.]]
+[[Category: Skehel JJ]]
-[[Category: Skehel, J.J.]]
+[[Category: Vogan EM]]
-[[Category: Vogan, E.M.]]
+[[Category: Wiley DC]]
-[[Category: Wiley, D.C.]]
-[[Category: MAN]]
-[[Category: NAG]]
-[[Category: aids]]
-[[Category: coat protein]]
-[[Category: envelope glycoprotein]]
-[[Category: gp120]]
-[[Category: siv]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:46:09 2007''