1rw1: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1rw1.gif|left|200px]]<br /><applet load="1rw1" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1rw1, resolution 1.02&Aring;" />
-'''YFFB (PA3664) PROTEIN'''<br />
-==Overview==
+==YFFB (PA3664) PROTEIN==
-BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an, uncharacterized protein of 13 kDa molecular weight with a marginal, sequence similarity to arsenate reductase from Escherichia coli. The, crystal structure determination of YffB was undertaken as part of a, structural genomics effort in order to assist with the functional, assignment of the protein. RESULTS: The structure was determined at 1.0 A, resolution by single-wavelength anomalous diffraction. The fold is very, similar to that of arsenate reductase, which is an extension of the, thioredoxin fold. CONCLUSION: Given the conservation of the functionally, important residues and the ability to bind glutathione, YffB is likely to, function as a GSH-dependent thiol reductase.
+<StructureSection load='1rw1' size='340' side='right'caption='[[1rw1]], [[Resolution|resolution]] 1.02&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1rw1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RW1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.02&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rw1 OCA], [https://pdbe.org/1rw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rw1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rw1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9HXX5_PSEAE Q9HXX5_PSEAE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rw1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rw1 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. RESULTS: The structure was determined at 1.0 A resolution by single-wavelength anomalous diffraction. The fold is very similar to that of arsenate reductase, which is an extension of the thioredoxin fold. CONCLUSION: Given the conservation of the functionally important residues and the ability to bind glutathione, YffB is likely to function as a GSH-dependent thiol reductase.
-==About this Structure==
+Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function.,Teplyakov A, Pullalarevu S, Obmolova G, Doseeva V, Galkin A, Herzberg O, Dauter M, Dauter Z, Gilliland GL BMC Struct Biol. 2004 Mar 8;4:5. PMID:15102337<ref>PMID:15102337</ref>
-RW1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with IPA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RW1 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function., Teplyakov A, Pullalarevu S, Obmolova G, Doseeva V, Galkin A, Herzberg O, Dauter M, Dauter Z, Gilliland GL, BMC Struct Biol. 2004 Mar 8;4:5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15102337 15102337]
+</div>
-[[Category: Pseudomonas aeruginosa pao1]]
+<div class="pdbe-citations 1rw1" style="background-color:#fffaf0;"></div>
-[[Category: Single protein]]
+== References ==
-[[Category: Dauter, M.]]
+<references/>
-[[Category: Dauter, Z.]]
+__TOC__
-[[Category: Doseeva, V.]]
+</StructureSection>
-[[Category: Galkin, A.]]
+[[Category: Large Structures]]
-[[Category: Gilliland, G.L.]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Herzberg, O.]]
+[[Category: Dauter M]]
-[[Category: Obmolova, G.]]
+[[Category: Dauter Z]]
-[[Category: Pullalarevu, S.]]
+[[Category: Doseeva V]]
-[[Category: S2F, Structure.2.Function.Project.]]
+[[Category: Galkin A]]
-[[Category: Teplyakov, A.]]
+[[Category: Gilliland GL]]
-[[Category: IPA]]
+[[Category: Herzberg O]]
-[[Category: s2f]]
+[[Category: Obmolova G]]
-[[Category: structural genomics]]
+[[Category: Pullalarevu S]]
-[[Category: structure 2 function project]]
+[[Category: Teplyakov A]]
-[[Category: thioredoxin fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:23:13 2007''