1b6v: Difference between revisions

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-[[Image:1b6v.jpg|left|200px]]<br /><applet load="1b6v" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1b6v, resolution 2.00&Aring;" />
-'''CRYSTAL STRUCTURE OF A HYBRID BETWEEN RIBONUCLEASE A AND BOVINE SEMINAL RIBONUCLEASE'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF A HYBRID BETWEEN RIBONUCLEASE A AND BOVINE SEMINAL RIBONUCLEASE==
-A variant of bovine pancreatic ribonuclease A has been prepared with seven, amino acid substitutions (Q55K, N62K, A64T, Y76K, S80R, E111G, N113K)., These substitutions recreate in RNase A the basic surface found in bovine, seminal RNase, a homologue of pancreatic RNase that diverged some 35, million years ago. Substitution of a portion of this basic surface, (positions 55, 62, 64, 111 and 113) enhances the immunosuppressive, activity of the RNase variant, activity found in native seminal RNase, while substitution of another portion (positions 76 and 80) attenuates the, activity. Further, introduction of Gly at position 111 has been shown to, increase the catalytic activity of RNase against double-stranded RNA. The, variant and the wild-type (recombinant) protein were crystallized and, their structures determined to a resolution of 2.0 A. Each of the mutated, amino acids is seen in the electron density map. The main change observed, in the mutant structure compared with the wild-type is the region, encompassing residues 16-22, where the structure is more disordered. This, loop is the region where the polypeptide chain of RNase A is cleaved by, subtilisin to form RNase S, and undergoes conformational change to allow, residues 1-20 of the RNase to swap between subunits in the covalent, seminal RNase dimer.
+<StructureSection load='1b6v' size='340' side='right'caption='[[1b6v]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1b6v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B6V FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b6v OCA], [https://pdbe.org/1b6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b6v RCSB], [https://www.ebi.ac.uk/pdbsum/1b6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b6v ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/1b6v_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b6v ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A variant of bovine pancreatic ribonuclease A has been prepared with seven amino acid substitutions (Q55K, N62K, A64T, Y76K, S80R, E111G, N113K). These substitutions recreate in RNase A the basic surface found in bovine seminal RNase, a homologue of pancreatic RNase that diverged some 35 million years ago. Substitution of a portion of this basic surface (positions 55, 62, 64, 111 and 113) enhances the immunosuppressive activity of the RNase variant, activity found in native seminal RNase, while substitution of another portion (positions 76 and 80) attenuates the activity. Further, introduction of Gly at position 111 has been shown to increase the catalytic activity of RNase against double-stranded RNA. The variant and the wild-type (recombinant) protein were crystallized and their structures determined to a resolution of 2.0 A. Each of the mutated amino acids is seen in the electron density map. The main change observed in the mutant structure compared with the wild-type is the region encompassing residues 16-22, where the structure is more disordered. This loop is the region where the polypeptide chain of RNase A is cleaved by subtilisin to form RNase S, and undergoes conformational change to allow residues 1-20 of the RNase to swap between subunits in the covalent seminal RNase dimer.
-==About this Structure==
+Crystal structure of a hybrid between ribonuclease A and bovine seminal ribonuclease--the basic surface, at 2.0 A resolution.,Vatzaki EH, Allen SC, Leonidas DD, Trautwein-Fritz K, Stackhouse J, Benner SA, Acharya KR Eur J Biochem. 1999 Feb;260(1):176-82. PMID:10091597<ref>PMID:10091597</ref>
-B6V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B6V OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of a hybrid between ribonuclease A and bovine seminal ribonuclease--the basic surface, at 2.0 A resolution., Vatzaki EH, Allen SC, Leonidas DD, Trautwein-Fritz K, Stackhouse J, Benner SA, Acharya KR, Eur J Biochem. 1999 Feb;260(1):176-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10091597 10091597]
+</div>
+<div class="pdbe-citations 1b6v" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
-[[Category: Pancreatic ribonuclease]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Acharya KR]]
-[[Category: Acharya, K.R.]]
+[[Category: Allen SC]]
-[[Category: Allen, S.C.]]
+[[Category: Leonidas DD]]
-[[Category: Leonidas, D.D.]]
+[[Category: Vatzaki EH]]
-[[Category: Vatzaki, E.H.]]
-[[Category: molecular evolution]]
-[[Category: ribonuclease]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:24:33 2007''