8k0b: Difference between revisions
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The | ==Cryo-EM structure of TMEM63C== | ||
<StructureSection load='8k0b' size='340' side='right'caption='[[8k0b]], [[Resolution|resolution]] 3.56Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8k0b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8K0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8K0B FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.56Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8k0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8k0b OCA], [https://pdbe.org/8k0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8k0b RCSB], [https://www.ebi.ac.uk/pdbsum/8k0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8k0b ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CSC1_MOUSE CSC1_MOUSE] Acts as an osmosensitive cation channel preferentially activated upon hypotonic stress (PubMed:27045885, PubMed:32375046, PubMed:37945568). Enriched in mitochondria-ER contact sites where it may regulate the metabolite flux and organelles' morphologies in response to osmotic changes. In particular may regulate mitochondrial motility and function in motor neuron axons (By similarity). Required for the functional integrity of the kidney glomerular filtration barrier (By similarity).[UniProtKB:D3ZNF5][UniProtKB:Q9P1W3]<ref>PMID:27045885</ref> <ref>PMID:32375046</ref> <ref>PMID:37945568</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated in various physiological functions. Deficiency of these channels would cause many diseases including hearing loss. However, their structures and physiological roles are not yet well understood. In this study, we determine the cryo-electron microscopy (cryo-EM) structure of the mouse TMEM63C at 3.56 A, and revealed structural differences compared to TMEM63A, TMEM63B, and the plant orthologues OSCAs. Further structural guided mutagenesis and calcium imaging demonstrated the important roles of the coupling of TM0 and TM6 in channel activity. Additionally, we confirm that TMEM63C exists primarily as a monomer under physiological conditions, in contrast, TMEM63B is a mix of monomer and dimer in cells, suggesting that oligomerization is a regulatory mechanism for TMEM63 proteins. | |||
Cryo-EM structure of TMEM63C suggests it functions as a monomer.,Qin Y, Yu D, Wu D, Dong J, Li WT, Ye C, Cheung KC, Zhang Y, Xu Y, Wang Y, Shi YS, Dang S Nat Commun. 2023 Nov 9;14(1):7265. doi: 10.1038/s41467-023-42956-2. PMID:37945568<ref>PMID:37945568</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8k0b" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | |||
[[Category: Dang S]] | |||
[[Category: Dong J]] | |||
[[Category: Qin Y]] | |||
[[Category: Yu D]] | |||
Latest revision as of 12:56, 9 October 2024
Cryo-EM structure of TMEM63CCryo-EM structure of TMEM63C
Structural highlights
FunctionCSC1_MOUSE Acts as an osmosensitive cation channel preferentially activated upon hypotonic stress (PubMed:27045885, PubMed:32375046, PubMed:37945568). Enriched in mitochondria-ER contact sites where it may regulate the metabolite flux and organelles' morphologies in response to osmotic changes. In particular may regulate mitochondrial motility and function in motor neuron axons (By similarity). Required for the functional integrity of the kidney glomerular filtration barrier (By similarity).[UniProtKB:D3ZNF5][UniProtKB:Q9P1W3][1] [2] [3] Publication Abstract from PubMedThe TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated in various physiological functions. Deficiency of these channels would cause many diseases including hearing loss. However, their structures and physiological roles are not yet well understood. In this study, we determine the cryo-electron microscopy (cryo-EM) structure of the mouse TMEM63C at 3.56 A, and revealed structural differences compared to TMEM63A, TMEM63B, and the plant orthologues OSCAs. Further structural guided mutagenesis and calcium imaging demonstrated the important roles of the coupling of TM0 and TM6 in channel activity. Additionally, we confirm that TMEM63C exists primarily as a monomer under physiological conditions, in contrast, TMEM63B is a mix of monomer and dimer in cells, suggesting that oligomerization is a regulatory mechanism for TMEM63 proteins. Cryo-EM structure of TMEM63C suggests it functions as a monomer.,Qin Y, Yu D, Wu D, Dong J, Li WT, Ye C, Cheung KC, Zhang Y, Xu Y, Wang Y, Shi YS, Dang S Nat Commun. 2023 Nov 9;14(1):7265. doi: 10.1038/s41467-023-42956-2. PMID:37945568[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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