7lu2: Difference between revisions
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==X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 6== | ==X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 6== | ||
<StructureSection load='7lu2' size='340' side='right'caption='[[7lu2]]' scene=''> | <StructureSection load='7lu2' size='340' side='right'caption='[[7lu2]], [[Resolution|resolution]] 1.11Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LU2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7lu2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LU2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lu2 OCA], [https://pdbe.org/7lu2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lu2 RCSB], [https://www.ebi.ac.uk/pdbsum/7lu2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lu2 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.11Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lu2 OCA], [https://pdbe.org/7lu2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lu2 RCSB], [https://www.ebi.ac.uk/pdbsum/7lu2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lu2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cryo-cooling has been nearly universally adopted to mitigate X-ray damage and facilitate crystal handling in protein X-ray crystallography. However, cryo X-ray crystallographic data provide an incomplete window into the ensemble of conformations that is at the heart of protein function and energetics. Room-temperature (RT) X-ray crystallography provides accurate ensemble information, and recent developments allow conformational heterogeneity (the experimental manifestation of ensembles) to be extracted from single-crystal data. Nevertheless, high sensitivity to X-ray damage at RT raises concerns about data reliability. To systematically address this critical issue, increasingly X-ray-damaged high-resolution data sets (1.02-1.52 A resolution) were obtained from single proteinase K, thaumatin and lysozyme crystals at RT (277 K). In each case a modest increase in conformational heterogeneity with X-ray damage was observed. Merging data with different extents of damage (as is typically carried out) had negligible effects on conformational heterogeneity until the overall diffraction intensity decayed to approximately 70% of its initial value. These effects were compared with X-ray damage effects in cryo-cooled crystals by carrying out an analogous analysis of increasingly damaged proteinase K cryo data sets (0.9-1.16 A resolution). X-ray damage-associated heterogeneity changes were found that were not observed at RT. This property renders it difficult to distinguish real from artefactual conformations and to determine the conformational response to changes in temperature. The ability to acquire reliable heterogeneity information from single crystals at RT, together with recent advances in RT data collection at accessible synchrotron beamlines, provides a strong motivation for the widespread adoption of RT X-ray crystallography to obtain conformational ensemble information. | |||
Evaluating the impact of X-ray damage on conformational heterogeneity in room-temperature (277 K) and cryo-cooled protein crystals.,Yabukarski F, Doukov T, Mokhtari DA, Du S, Herschlag D Acta Crystallogr D Struct Biol. 2022 Aug 1;78(Pt 8):945-963. doi: , 10.1107/S2059798322005939. Epub 2022 Jul 14. PMID:35916220<ref>PMID:35916220</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7lu2" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Proteinase 3D structures|Proteinase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Parengyodontium album]] | |||
[[Category: Doukov T]] | [[Category: Doukov T]] | ||
[[Category: Herschlag D]] | [[Category: Herschlag D]] | ||
[[Category: Yabukarski F]] | [[Category: Yabukarski F]] | ||