4qi6: Difference between revisions

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-'''Unreleased structure'''
-The entry 4qi6 is ON HOLD  until 00 0001
+==Cellobiose dehydrogenase from Myriococcum thermophilum, MtCDH==
+<StructureSection load='4qi6' size='340' side='right'caption='[[4qi6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4qi6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_myriococcoides Thermothelomyces myriococcoides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QI6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi6 OCA], [https://pdbe.org/4qi6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qi6 RCSB], [https://www.ebi.ac.uk/pdbsum/4qi6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A9XK88_9PEZI A9XK88_9PEZI]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving cellobiose dehydrogenases (CDH) and copper-dependent lytic polysaccharide monooxygenases (LPMO); however, mechanistic studies have been hampered by the lack of structural information regarding CDH. CDH contains a haem-binding cytochrome (CYT) connected via a flexible linker to a flavin-dependent dehydrogenase (DH). Electrons are generated from cellobiose oxidation catalysed by DH and shuttled via CYT to LPMO. Here we present structural analyses that provide a comprehensive picture of CDH conformers, which govern the electron transfer between redox centres. Using structure-based site-directed mutagenesis, rapid kinetics analysis and molecular docking, we demonstrate that flavin-to-haem interdomain electron transfer (IET) is enabled by a haem propionate group and that rapid IET requires a closed CDH state in which the propionate is tightly enfolded by DH. Following haem reduction, CYT reduces LPMO to initiate oxygen activation at the copper centre and subsequent cellulose depolymerization.
-Authors: Tan, T.C., Gandini, R., Sygmund, C., Kittl, R., Haltrich, D., Ludwig, R., Hallberg, B.M., Divne, C.
+Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation.,Tan TC, Kracher D, Gandini R, Sygmund C, Kittl R, Haltrich D, Hallberg BM, Ludwig R, Divne C Nat Commun. 2015 Jul 7;6:7542. doi: 10.1038/ncomms8542. PMID:26151670<ref>PMID:26151670</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Haltrich, D]]
+<div class="pdbe-citations 4qi6" style="background-color:#fffaf0;"></div>
-[[Category: Divne, C]]
+== References ==
-[[Category: Gandini, R]]
+<references/>
-[[Category: Hallberg, B.M]]
+__TOC__
-[[Category: Ludwig, R]]
+</StructureSection>
-[[Category: Sygmund, C]]
+[[Category: Large Structures]]
-[[Category: Tan, T.C]]
+[[Category: Thermothelomyces myriococcoides]]
-[[Category: Kittl, R]]
+[[Category: Divne C]]
+[[Category: Gandini R]]
+[[Category: Hallberg BM]]
+[[Category: Haltrich D]]
+[[Category: Kittl R]]
+[[Category: Ludwig R]]
+[[Category: Sygmund C]]
+[[Category: Tan TC]]