3fz2: Difference between revisions

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-{{Seed}}
-[[Image:3fz2.jpg|left|200px]]
-<!--
+==Crystal structure of the tail terminator protein from phage lambda (gpU-D74A)==
-The line below this paragraph, containing "STRUCTURE_3fz2", creates the "Structure Box" on the page.
+<StructureSection load='3fz2' size='340' side='right'caption='[[3fz2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3fz2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FZ2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_3fz2|  PDB=3fz2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fz2 OCA], [https://pdbe.org/3fz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fz2 RCSB], [https://www.ebi.ac.uk/pdbsum/3fz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fz2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TTTP_LAMBD TTTP_LAMBD] Plays an essential role in tail assembly by capping the rapidly polymerizing tail once it has reached its requisite length and serving as the interaction surface for the completion protein.<ref>PMID:2150582</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/3fz2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fz2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The tail terminator protein (TrP) plays an essential role in phage tail assembly by capping the rapidly polymerizing tail once it has reached its requisite length and serving as the interaction surface for phage heads. Here, we present the 2.7-A crystal structure of a hexameric ring of gpU, the TrP of phage lambda. Using sequence alignment analysis and site-directed mutagenesis, we have shown that this multimeric structure is biologically relevant and we have delineated its functional surfaces. Comparison of the hexameric crystal structure with the solution structure of gpU that we previously solved using NMR spectroscopy shows large structural changes occurring upon multimerization and suggests a mechanism that allows gpU to remain monomeric at high concentrations on its own, yet polymerize readily upon contact with an assembled tail tube. The gpU hexamer displays several flexible loops that play key roles in head and tail binding, implying a role for disorder-to-order transitions in controlling assembly as has been observed with other lambda morphogenetic proteins. Finally, we have found that the hexameric structure of gpU is very similar to the structure of a putative TrP from a contractile phage tail even though it displays no detectable sequence similarity. This finding coupled with further bioinformatic investigations has led us to conclude that the TrPs of non-contractile-tailed phages, such as lambda, are evolutionarily related to those of contractile-tailed phages, such as P2 and Mu, and that all long-tailed phages may utilize a conserved mechanism for tail termination.
-===Crystal structure of the tail terminator protein from phage lambda (gpU-D74A)===
+The X-ray crystal structure of the phage lambda tail terminator protein reveals the biologically relevant hexameric ring structure and demonstrates a conserved mechanism of tail termination among diverse long-tailed phages.,Pell LG, Liu A, Edmonds L, Donaldson LW, Howell PL, Davidson AR J Mol Biol. 2009 Jun 26;389(5):938-51. Epub 2009 May 6. PMID:19426744<ref>PMID:19426744</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_19426744}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3fz2" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 19426744 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_19426744}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Escherichia virus Lambda]]
-FZ2 is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FZ2 OCA].
+[[Category: Large Structures]]
+[[Category: Davidson AR]]
-==Reference==
+[[Category: Donaldson LW]]
-<ref group="xtra">PMID:19426744</ref><references group="xtra"/>
+[[Category: Edmonds E]]
-[[Category: Enterobacteria phage lambda]]
+[[Category: Howell PL]]
-[[Category: Davidson, A R.]]
+[[Category: Liu A]]
-[[Category: Donaldson, L W.]]
+[[Category: Pell LG]]
-[[Category: Edmonds, E.]]
-[[Category: Howell, P L.]]
-[[Category: Liu, A.]]
-[[Category: Pell, L G.]]
-[[Category: Mixed alpha-beta fold]]
-[[Category: Viral protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 27 15:20:37 2009''