3bgw: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3bgw.jpg|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_3bgw", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3bgw|  PDB=3bgw  |  SCENE=  }}
-'''The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase'''
+==The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase==
+<StructureSection load='3bgw' size='340' side='right'caption='[[3bgw]], [[Resolution|resolution]] 3.91&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3bgw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_SPP1 Bacillus phage SPP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BGW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.91&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bgw OCA], [https://pdbe.org/3bgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bgw RCSB], [https://www.ebi.ac.uk/pdbsum/3bgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bgw ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q38152_BPSPP Q38152_BPSPP]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/3bgw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bgw ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Helicases are essential enzymes for DNA replication, a fundamental process in all living organisms. The DnaB family are hexameric replicative helicases that unwind duplex DNA and coordinate with RNA primase and other proteins at the replication fork in prokaryotes. Here, we report the full-length crystal structure of G40P, a DnaB family helicase. The hexamer complex reveals an unusual architectural feature and a new type of assembly mechanism. The hexamer has two tiers: a three-fold symmetric N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with two different conformations, termed cis and trans, come together to provide a topological solution for the dual symmetry within a hexamer. Structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. This study provides insights into the structural and functional interplay between G40P helicase and DnaG primase.
-==Overview==
+The structure of a DnaB-family replicative helicase and its interactions with primase.,Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:18157148<ref>PMID:18157148</ref>
-Helicases are essential enzymes for DNA replication, a fundamental process in all living organisms. The DnaB family are hexameric replicative helicases that unwind duplex DNA and coordinate with RNA primase and other proteins at the replication fork in prokaryotes. Here, we report the full-length crystal structure of G40P, a DnaB family helicase. The hexamer complex reveals an unusual architectural feature and a new type of assembly mechanism. The hexamer has two tiers: a three-fold symmetric N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with two different conformations, termed cis and trans, come together to provide a topological solution for the dual symmetry within a hexamer. Structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. This study provides insights into the structural and functional interplay between G40P helicase and DnaG primase.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-BGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spp1 Bacillus phage spp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGW OCA].
+</div>
+<div class="pdbe-citations 3bgw" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18157148 18157148]
+*[[Helicase 3D structures|Helicase 3D structures]]
-[[Category: Bacillus phage spp1]]
+== References ==
-[[Category: Single protein]]
+<references/>
-[[Category: Chen, X S.]]
+__TOC__
-[[Category: Holden, L G.]]
+</StructureSection>
-[[Category: Klein, M G.]]
+[[Category: Bacillus phage SPP1]]
-[[Category: Tokonzaba, E.]]
+[[Category: Large Structures]]
-[[Category: Wang, G.]]
+[[Category: Chen XS]]
-[[Category: Zhang, Y.]]
+[[Category: Holden LG]]
-[[Category: Atpase]]
+[[Category: Klein MG]]
-[[Category: Replication]]
+[[Category: Tokonzaba E]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:45:01 2008''
+[[Category: Wang G]]
+[[Category: Zhang Y]]