2x8r: Difference between revisions

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-[[Image:2x8r.png|left|200px]]
-{{STRUCTURE_2x8r|  PDB=2x8r  |  SCENE=  }}
+==The structure of a family GH25 lysozyme from Aspergillus fumigatus==
+<StructureSection load='2x8r' size='340' side='right'caption='[[2x8r]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2x8r]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8r OCA], [https://pdbe.org/2x8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8r RCSB], [https://www.ebi.ac.uk/pdbsum/2x8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A4DA29_ASPFU A4DA29_ASPFU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8r_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8r ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lysins are important biomolecules which cleave the bacterial cell-wall polymer peptidoglycan. They are finding increasing commercial and medical application. In order to gain an insight into the mechanism by which these enzymes operate, the X-ray structure of a CAZy family GH25 ;lysozyme' from Aspergillus fumigatus was determined. This is the first fungal structure from the family and reveals a modified alpha/beta-barrel-like fold in which an eight-stranded beta-barrel is flanked by three alpha-helices. The active site lies toward the bottom of a negatively charged pocket and its layout has much in common with other solved members of the GH25 and related GH families. A conserved active-site DXE motif may be implicated in catalysis, lending further weight to the argument that this glycoside hydrolase family operates via a ;substrate-assisted' catalytic mechanism.
-===The structure of a family GH25 lysozyme from Aspergillus fumigatus===
+The structure of a family GH25 lysozyme from Aspergillus fumigatus.,Korczynska JE, Danielsen S, Schagerlof U, Turkenburg JP, Davies GJ, Wilson KS, Taylor EJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt, 9):973-7. Epub 2010 Aug 21. PMID:20823508<ref>PMID:20823508</ref>
-{{ABSTRACT_PUBMED_20823508}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2x8r" style="background-color:#fffaf0;"></div>
-[[2x8r]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8R OCA].
 ==See Also==
-*[[Hen Egg-White (HEW) Lysozyme|Hen Egg-White (HEW) Lysozyme]]
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:020823508</ref><references group="xtra"/>
+__TOC__
-[[Category: Aspergillus fumigatus]]
+</StructureSection>
-[[Category: Lysozyme]]
+[[Category: Aspergillus fumigatus Af293]]
-[[Category: Danielsen, S.]]
+[[Category: Large Structures]]
-[[Category: Davies, G J.]]
+[[Category: Danielsen S]]
-[[Category: Korczynska, J E.]]
+[[Category: Davies GJ]]
-[[Category: Schagerlof, U.]]
+[[Category: Korczynska JE]]
-[[Category: Taylor, E J.]]
+[[Category: Schagerlof U]]
-[[Category: Turkenburg, J P.]]
+[[Category: Taylor EJ]]
-[[Category: Wilson, K S.]]
+[[Category: Turkenburg JP]]
-[[Category: Dxe motif]]
+[[Category: Wilson KS]]
-[[Category: Endo-n-acetylmuramidase]]
-[[Category: Hydrolase]]
-[[Category: Peptidoglycan cleavage]]