2q8a: Difference between revisions

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-{{Seed}}
-[[Image:2q8a.png|left|200px]]
-<!--
+==Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody==
-The line below this paragraph, containing "STRUCTURE_2q8a", creates the "Structure Box" on the page.
+<StructureSection load='2q8a' size='340' side='right'caption='[[2q8a]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2q8a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q8A FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q8a OCA], [https://pdbe.org/2q8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q8a RCSB], [https://www.ebi.ac.uk/pdbsum/2q8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q8a ProSAT]</span></td></tr>
-{{STRUCTURE_2q8a|  PDB=2q8a  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7KQK5_PLAF7 Q7KQK5_PLAF7]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q8/2q8a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q8a ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Identifying functionally critical regions of the malaria antigen AMA1 (apical membrane antigen 1) is necessary to understand the significance of the polymorphisms within this antigen for vaccine development. The crystal structure of AMA1 in complex with the Fab fragment of inhibitory monoclonal antibody 1F9 reveals that 1F9 binds to the AMA1 solvent-exposed hydrophobic trough, confirming its importance. 1F9 uses the heavy and light chain complementarity-determining regions (CDRs) to wrap around the polymorphic loops adjacent to the trough, but uses a ridge of framework residues to bind to the hydrophobic trough. The resulting 1F9-AMA1-combined buried surface of 2,470 A(2) is considerably larger than previously reported Fab-antigen interfaces. Mutations of polymorphic AMA1 residues within the 1F9 epitope disrupt 1F9 binding and dramatically reduce the binding of affinity-purified human antibodies. Moreover, 1F9 binding to AMA1 is competed by naturally acquired human antibodies, confirming that the 1F9 epitope is a frequent target of immunological attack.
-===Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody===
+Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody.,Coley AM, Gupta A, Murphy VJ, Bai T, Kim H, Foley M, Anders RF, Batchelor AH PLoS Pathog. 2007 Sep 7;3(9):1308-19. PMID:17907804<ref>PMID:17907804</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_17907804}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2q8a" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 17907804 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17907804}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-Q8A is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q8A OCA].
-==Reference==
-<ref group="xtra">PMID:17907804</ref><references group="xtra"/>
 [[Category: Mus musculus]]
-[[Category: Plasmodium falciparum]]
+[[Category: Plasmodium falciparum 3D7]]
-[[Category: Anders, R F.]]
+[[Category: Anders RF]]
-[[Category: Batchelor, A H.]]
+[[Category: Batchelor AH]]
-[[Category: Gupta, A.]]
+[[Category: Gupta A]]
-[[Category: Murphy, V J.]]
+[[Category: Murphy VJ]]
-[[Category: Antigen-antibody complex]]
-[[Category: Immune system]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:37:18 2009''