2gx2: Difference between revisions

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-{{Seed}}
-[[Image:2gx2.png|left|200px]]
-<!--
+==Crystal structural and functional analysis of GFP-like fluorescent protein Dronpa==
-The line below this paragraph, containing "STRUCTURE_2gx2", creates the "Structure Box" on the page.
+<StructureSection load='2gx2' size='340' side='right'caption='[[2gx2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2gx2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GX2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-{{STRUCTURE_2gx2|  PDB=2gx2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gx2 OCA], [https://pdbe.org/2gx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gx2 RCSB], [https://www.ebi.ac.uk/pdbsum/2gx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gx2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/2gx2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gx2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, Ando and his colleagues developed a new green fluorescent protein Dronpa, which possesses the unique photochromic property of being photoswitchable in a non-destructive manner. To better understand this mechanism, we determined the crystal structures of a new GFP Dronpa and its mutant C62S, at 1.9 Angstroms and 1.8 Angstroms, respectively. Determination of the structures demonstrates that a unique hydrogen-bonding network and the sulfur atom of the chromophore are critical to the photoswitching property of Dronpa. Reversible photoswitching was lost in cells expressing the Dronpa-C62S upon repetitive irradiation compared to the native protein. Structural and mutational analyses reveal the chemical basis for the functional properties of photoswitchable fluorescent proteins and provide the basis for subsequent coherent engineering of this subfamily of Dronpa homologs.
-===Crystal structural and functional analysis of GFP-like fluorescent protein Dronpa===
+Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S.,Nam KH, Kwon OY, Sugiyama K, Lee WH, Kim YK, Song HK, Kim EE, Park SY, Jeon H, Hwang KY Biochem Biophys Res Commun. 2007 Mar 23;354(4):962-7. Epub 2007 Jan 24. PMID:17276392<ref>PMID:17276392</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2gx2" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_17276392}}, adds the Publication Abstract to the page
+*[[Dronpa|Dronpa]]
-(as it appears on PubMed at http://www.pubmed.gov), where 17276392 is the PubMed ID number.
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
--->
+== References ==
-{{ABSTRACT_PUBMED_17276392}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-GX2 is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GX2 OCA].
+[[Category: Echinophyllia sp. SC22]]
+[[Category: Large Structures]]
-==Reference==
+[[Category: Hwang KY]]
-<ref group="xtra">PMID:17276392</ref><references group="xtra"/>
+[[Category: Nam K-H]]
-[[Category: Echinophyllia sp. sc22]]
+[[Category: Park S-Y]]
-[[Category: Hwang, K Y.]]
+[[Category: Sugiyama K]]
-[[Category: Nam, K H.]]
-[[Category: Park, S Y.]]
-[[Category: Sugiyama, K.]]
-[[Category: Fluorescence protein]]
-[[Category: Luminescent protein]]
-[[Category: Photoswiching]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:11:24 2009''