1xau: Difference between revisions

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-[[Image:1xau.jpg|left|200px]]<br /><applet load="1xau" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1xau, resolution 1.80&Aring;" />
-'''STRUCTURE OF THE BTLA ECTODOMAIN'''<br />
-==About this Structure==
+==STRUCTURE OF THE BTLA ECTODOMAIN==
-XAU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAU OCA].
+<StructureSection load='1xau' size='340' side='right'caption='[[1xau]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1xau]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xau OCA], [https://pdbe.org/1xau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xau RCSB], [https://www.ebi.ac.uk/pdbsum/1xau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xau ProSAT], [https://www.topsan.org/Proteins/MCSG/1xau TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/BTLA_MOUSE BTLA_MOUSE] Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The B and T lymphocyte attenuator (BTLA) appears to act as a negative regulator of T cell activation and growth. BTLA specifically interacts with herpesvirus entry mediator (HVEM), a member of the TNFR family. Herein, we have undertaken surface plasmon resonance studies to quantitatively assess BTLA and HVEM ectodomain interactions. We find that soluble BALB/cJ BTLA engages HVEM with an equilibrium affinity of 0.97+/-0.19 microM while the C57BL/6 BTLA binds slightly better with an equilibrium affinity of 0.42+/-0.06 microM. Despite its lower affinity for HVEM, the kinetic half-life of BALB/cJ BTLA complexes are twice as long as observed for C57BL/6 BTLA (4 vs 2 s). To further explore these interactions, we solved the crystal structure of a murine BTLA (BALB/cJ) ectodomain at 1.8-A resolution, revealing a beta sandwich fold with strong similarity to I-set members of the Ig superfamily. Using a structure-based mutagenesis strategy, we then examined the individual contributions of 26 BTLA surface-exposed residues toward HVEM binding. Four single-site substitutions were identified that decrease HVEM binding below detectable levels and two that decrease binding by more than half. All six of these cluster at the edge of the beta sandwich in a membrane distal patch formed primarily from the A and G strands. This patch falls within the contacting surface recently revealed in the crystal structure of the human BTLA-HVEM cocomplex. The critical binding residues identified here are highly conserved across species, suggesting that BTLA employs a conserved binding mode for HVEM recognition.
+Structural determinants of herpesvirus entry mediator recognition by murine B and T lymphocyte attenuator.,Nelson CA, Fremont MD, Sedy JR, Norris PS, Ware CF, Murphy KM, Fremont DH J Immunol. 2008 Jan 15;180(2):940-7. PMID:18178834<ref>PMID:18178834</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1xau" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Single protein]]
+[[Category: Fremont DH]]
-[[Category: Fremont, D H.]]
+[[Category: Nelson CA]]
-[[Category: MCSG, Midwest Center for Structural Genomics.]]
-[[Category: Nelson, C A.]]
-[[Category: CD]]
-[[Category: beta sandwich]]
-[[Category: ig domain]]
-[[Category: mcsg]]
-[[Category: midwest center for structural genomics]]
-[[Category: protein structure initiative]]
-[[Category: psi]]
-[[Category: structural genomics]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:50 2008''