1kio: Difference between revisions

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-{{Seed}}
-[[Image:1kio.png|left|200px]]
-<!--
+==SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]==
-The line below this paragraph, containing "STRUCTURE_1kio", creates the "Structure Box" on the page.
+<StructureSection load='1kio' size='340' side='right'caption='[[1kio]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1kio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistocerca_gregaria Schistocerca gregaria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KIO FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kio OCA], [https://pdbe.org/1kio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kio RCSB], [https://www.ebi.ac.uk/pdbsum/1kio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kio ProSAT]</span></td></tr>
-{{STRUCTURE_1kio|  PDB=1kio  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SGP1_SCHGR SGP1_SCHGR] In vitro, SGPI-1/SGCI is active against alpha-chymotrypsin and trypsin while SGPI-2/SGTI is active against alpha-chymotrypsin and pancreatic elastase.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit different specificities towards target proteinases, where SGCI is a good chymotrypsin inhibitor, its mutant is a potent trypsin inhibitor, and SGTI inhibits both proteinases weakly. Interestingly, SGTI is a much better inhibitor of insect proteinases than of the mammalian ones used in common assays. All three molecules have a similar fold composed from three antiparallel beta-pleated sheets with three disulfide bridges. The proteinase binding loop has a somewhat distinct geometry in all three peptides. Moreover, the stabilization of the structure is different in SGCI and SGTI. Proton-deuterium exchange experiments are indicative of a highly rigid core in SGTI but not in SGCI. We suggest that the observed structural properties play a significant role in the specificity of these inhibitors.
-===SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]===
+Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria.,Gaspari Z, Patthy A, Graf L, Perczel A Eur J Biochem. 2002 Jan;269(2):527-37. PMID:11856311<ref>PMID:11856311</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1kio" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_11856311}}, adds the Publication Abstract to the page
+*[[Chymotrypsin inhibitor 3D structures|Chymotrypsin inhibitor 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 11856311 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_11856311}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-KIO is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA].
+[[Category: Schistocerca gregaria]]
+[[Category: Gaspari Z]]
-==Reference==
+[[Category: Graf L]]
-Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria., Gaspari Z, Patthy A, Graf L, Perczel A, Eur J Biochem. 2002 Jan;269(2):527-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11856311 11856311]
+[[Category: Patthy A]]
-[[Category: Single protein]]
+[[Category: Perczel A]]
-[[Category: Gaspari, Z.]]
-[[Category: Graf, L.]]
-[[Category: Patthy, A.]]
-[[Category: Perczel, A.]]
-[[Category: Modified specificity]]
-[[Category: Protease inhibitor]]
-[[Category: Specificity]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 10:24:09 2008''