1ie6: Difference between revisions

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 ==SOLUTION STRUCTURE OF IMPERATOXIN A==
-<StructureSection load='1ie6' size='340' side='right' caption='[[1ie6]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1ie6' size='340' side='right'caption='[[1ie6]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ie6]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ie6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pandinus_imperator Pandinus imperator]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE6 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ie6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie6 PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
-<table>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie6 OCA], [https://pdbe.org/1ie6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAIMP_PANIM CAIMP_PANIM] This toxin affects the activity of ryanodine receptors 1, 2 and 3 (RyR1, RyR2 and RyR3) (PubMed:11867448, PubMed:1334561, PubMed:9565405). At lower concentrations the toxin increases full openings of the RyRs, and at higher concentrations it inhibits full openings and induces openings to subconductance levels (30% of the full conductance state) and reduces the number of full conductance openings (PubMed:27114612, PubMed:9565405). The different actions may be attributed to the toxins binding at different sites on the RyRs, with binding at a high-affinity site mediating the increase in full openings and the induction of subconductance states evoked upon binding to a lower-affinity site (PubMed:14699105). Furthermore, it triggers calcium release from sarcoplasmic vesicles (11.7 nM are enough to induce a sharp release, and 70% of the total calcium is released after toxin (100 nM) addition) probably by acting as a cell-penetrating peptide (CPP) (PubMed:1334561, PubMed:27114612). In addition, it has been shown to dose-dependently stimulate ryanodine binding to RyR1 (EC(50)=8.7 nM) (PubMed:27114612). It also augments the bell-shaped calcium-[3H]ryanodine binding curve that is maximal at about 10 uM calcium concentration (PubMed:27114612). It binds a different site as ryanodine (PubMed:9565405). It acts synergistically with caffeine (By similarity). In vivo, intracerebroventricular injection into mice induces neurotoxic symptoms, followed by death (By similarity).[UniProtKB:A0A1L4BJ42][UniProtKB:B8QG00][UniProtKB:P60254]<ref>PMID:11867448</ref> <ref>PMID:1334561</ref> <ref>PMID:14699105</ref> <ref>PMID:9565405</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
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 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1ie6" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Kim, D H.]]
+[[Category: Large Structures]]
-[[Category: Kim, J I.]]
+[[Category: Pandinus imperator]]
-[[Category: Lee, C W.]]
+[[Category: Kim DH]]
-[[Category: Sato, K.]]
+[[Category: Kim JI]]
-[[Category: Shimada, I.]]
+[[Category: Lee CW]]
-[[Category: Takahashi, H.]]
+[[Category: Sato K]]
-[[Category: Takeuchi, K.]]
+[[Category: Shimada I]]
-[[Category: Inhibitory cystine knot]]
+[[Category: Takahashi H]]
-[[Category: Toxin]]
+[[Category: Takeuchi K]]
-[[Category: Triple stranded antiparellel beta-sheet]]