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==== | ==Structure of human soluble guanylate cyclase in the cinciguat-bound activated state== | ||
<StructureSection load='7d9u' size='340' side='right'caption='[[7d9u]]' scene=''> | <StructureSection load='7d9u' size='340' side='right'caption='[[7d9u]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7d9u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D9U FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d9u OCA], [https://pdbe.org/7d9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d9u RCSB], [https://www.ebi.ac.uk/pdbsum/7d9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d9u ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Z90:4-({(4-CARBOXYBUTYL)[2-(2-{[4-(2-PHENYLETHYL)BENZYL]OXY}PHENYL)ETHYL]AMINO}METHYL)BENZOIC+ACID'>Z90</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d9u OCA], [https://pdbe.org/7d9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d9u RCSB], [https://www.ebi.ac.uk/pdbsum/7d9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d9u ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/GCYB1_HUMAN GCYB1_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Soluble guanylate cyclase (sGC) is the receptor for nitric oxide (NO) in human. It is an important validated drug target for cardiovascular diseases. sGC can be pharmacologically activated by stimulators and activators. However, the detailed structural mechanisms, through which sGC is recognized and positively modulated by these drugs at high spacial resolution, are poorly understood. Here, we present cryo-electron microscopy structures of human sGC in complex with NO and sGC stimulators, YC-1 and riociguat, and also in complex with the activator cinaciguat. These structures uncover the molecular details of how stimulators interact with residues from both beta H-NOX and CC domains, to stabilize sGC in the extended active conformation. In contrast, cinaciguat occupies the haem pocket in the beta H-NOX domain and sGC shows both inactive and active conformations. These structures suggest a converged mechanism of sGC activation by pharmacological compounds. | |||
Activation mechanism of human soluble guanylate cyclase by stimulators and activators.,Liu R, Kang Y, Chen L Nat Commun. 2021 Sep 17;12(1):5492. doi: 10.1038/s41467-021-25617-0. PMID:34535643<ref>PMID:34535643</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7d9u" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Guanylate cyclase 3D structures|Guanylate cyclase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Chen L]] | ||
[[Category: Kang Y]] | |||
[[Category: Liu R]] | |||
Latest revision as of 22:31, 29 May 2024
Structure of human soluble guanylate cyclase in the cinciguat-bound activated stateStructure of human soluble guanylate cyclase in the cinciguat-bound activated state
Structural highlights
FunctionPublication Abstract from PubMedSoluble guanylate cyclase (sGC) is the receptor for nitric oxide (NO) in human. It is an important validated drug target for cardiovascular diseases. sGC can be pharmacologically activated by stimulators and activators. However, the detailed structural mechanisms, through which sGC is recognized and positively modulated by these drugs at high spacial resolution, are poorly understood. Here, we present cryo-electron microscopy structures of human sGC in complex with NO and sGC stimulators, YC-1 and riociguat, and also in complex with the activator cinaciguat. These structures uncover the molecular details of how stimulators interact with residues from both beta H-NOX and CC domains, to stabilize sGC in the extended active conformation. In contrast, cinaciguat occupies the haem pocket in the beta H-NOX domain and sGC shows both inactive and active conformations. These structures suggest a converged mechanism of sGC activation by pharmacological compounds. Activation mechanism of human soluble guanylate cyclase by stimulators and activators.,Liu R, Kang Y, Chen L Nat Commun. 2021 Sep 17;12(1):5492. doi: 10.1038/s41467-021-25617-0. PMID:34535643[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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